Functional development of olfactory nerve-related neural circuits in the embryonic chick forebrain revealed by voltage-sensitive dye imaging.
Functional development of olfactory nerve-related neural circuits in the embryonic chick forebrain revealed by voltage-sensitive dye imaging.
复制标题
通过电压敏感染料成像揭示胚胎鸡前脑中嗅觉神经相关神经回路的功能发育。
DOI：
10.1111/ejn.15788
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发表时间：
2022
影响因子：
3.4
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Sato;K. and Momose-Sato;Y.
采用电压敏感吸收染料NK 2761对鸡胚嗅觉系统进行多位点光学记录，并在前脑中检查嗅觉神经（N.I）相关神经回路的功能发育。N. I在胚胎8-12天（E8-E12）阶段的N.I-嗅球（OB）-前脑制备物中引起神经反应。在E11阶段，我们在功能上识别了从OB到前脑投射的两个回路。第一回路穿过前脑的腹侧并在背尾方向上传播，而第二回路穿过背侧到达第一回路。药理学实验表明，N-甲基-D-天冬氨酸（NMDA）受体功能在这些回路中的感觉信息传递中更为重要。研究了N.I相关回路的功能发育，结果显示腹侧回路的产生早于背侧回路。腹侧回路中的神经反应在正常生理溶液中从E9阶段检测到，在无Mg 2+溶液中从E8阶段检测到，其激活了NMDA受体功能。在E10阶段，除了腹侧反应外，背侧回路中的神经反应被清楚地识别。我们试图通过比较光学信号幅度的轮廓线图与先前报道的神经解剖学数据来确定前脑中继核的可能候选者。结果表明，在个体发育过程中，从外周到腭下的N.I相关神经回路的功能成熟早于那些到腭的神经回路。
Multiple‐site optical recordings with NK2761, a voltage‐sensitive absorption dye, were applied to the embryonic chick olfactory system, and the functional development of olfactory nerve (N.I)‐related neural circuits was examined in the forebrain. The stimulation of the N. I elicited neural responses in N.I‐olfactory bulb (OB)‐forebrain preparations at the embryonic 8–12 day (E8‐E12) stages. At the E11 stage, we functionally identified two circuits projecting from the OB to the forebrain. The first circuit passed through the ventral side of the forebrain and spread in the dorso‐caudal direction, whereas the second circuit passed through the dorsal side to the first circuit. Pharmacological experiments showed that N‐methyl‐D‐aspartate (NMDA) receptor function was more significant for the transfer of sensory information in these circuits. The functional development of N.I‐related circuits was investigated, and the results obtained revealed that the ventral circuit was generated earlier than the dorsal circuit. Neural responses in the ventral circuit were detected from the E9 stage in normal physiological solution and the E8 stage in Mg2+‐free solution, which activated NMDA receptor function. At the E10 stage, neural responses in the dorsal circuit were clearly recognised in addition to ventral responses. We attempted to identify possible candidates for relay nuclei in the forebrain by comparing contour line maps of the optical signal amplitude with previously reported neuroanatomical data. The results suggest that N.I‐related neural circuits from the periphery to the subpallium functionally mature earlier than those to the pallium during ontogenesis.