A-type procyanidins from litchi pericarp ameliorate hyperglycaemia by regulating hepatic and muscle glucose metabolism in streptozotocin (STZ)-induced diabetic mice fed with high fat diet
A-type procyanidins from litchi pericarp ameliorate hyperglycaemia by regulating hepatic and muscle glucose metabolism in streptozotocin (STZ)-induced diabetic mice fed with high fat diet
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DOI：
10.1016/j.jff.2016.08.010
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发表时间：
2016-12-01
影响因子：
5.6
通讯作者：
Sun, Zhida
中科院分区：
文献类型：
--
作者：
Li, Xiaopeng;Sui, Yong;Sun, Zhida
荔枝果皮富含a型原花青素。本研究评价了a型低聚原花青素（A-OPC）的预防和抗糖尿病作用。结果表明，A-OPC比b型低聚原花青素（B-OPC）更有效。AMP活化蛋白ldnase （AMPK）在肝脏和骨骼肌中活化。a - opc和B-OPC治疗后，葡萄糖激酶（GK）、肝脏葡萄糖转运蛋白2 （GLUT2）、骨骼肌葡萄糖转运蛋白4 （GLUT4）和胰岛素受体a （INSR）蛋白水平均显著升高（p < 0.05）。肝脏和骨骼肌中磷酸果糖激酶（PFK）、丙酮酸激酶（PK）和骨骼肌中丙酮酸脱氢酶（PDH）的糖酵解关键基因表达量显著上调，而糖异生、磷酸烯醇丙酮酸羧激酶（PEPCK）和葡萄糖-6-磷酸酶（G-6-Pase）的限制性基因表达量下调。A-OPC和B-OPC均通过抑制肝脏中葡萄糖的产生、调节葡萄糖转运系统相关蛋白的表达和增加糖酵解来改善葡萄糖稳态。(C) 2016 Elsevier Ltd.版权所有。
A-type procyanidins is rich in litchi pericarp. This study evaluated the prevention and anti diabetic effect of A-type oligomeric procyanidins (A-OPC). The results showed A-OPC was more effective than B-type oligomeric procyanidins (B-OPC) of lotus seedpod. AMP activated protein ldnase (AMPK) was activated in liver and skeletal muscle. The protein levels of glucokinase (GK), glucose transporter type 2 (GLUT2, in liver), glucose transporter type 4 (GLUT4, in skeletal muscle) and insulin receptor a (INSR) improved significantly with treatment of A-OPC or B-OPC (p < 0.05). Furthermore, the glycolytic key gene expressions of phosphofructokinase (PFK), pyruvate kinase (PK) in liver and skeletal muscle and pyruvate dehydrogenase (PDH) in skeletal muscle were notably up-regulated, while gene expressions, rate-limiting enzymes of gluconeogenesis, of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G-6-Pase) were down-regulated. Both A-OPC and B-OPC improved glucose homeostasis by inhibiting the glucose production in liver, regulating the expression of proteins involved in glucose transport system and increasing glycolysis. (C) 2016 Elsevier Ltd. All rights reserved.