Changes in the fine structure of mouse gastric epithelium produced by ethanol and urea.
Changes in the fine structure of mouse gastric epithelium produced by ethanol and urea.
复制标题
乙醇和尿素产生的小鼠胃上皮精细结构的变化。
DOI：
10.1016/s0016-5085(19)32927-0
复制
发表时间：
1974
期刊：
影响因子：
29.4
通讯作者：
J. Kirchner
中科院分区：
文献类型：
--
作者：
G. Eastwood;J. Kirchner
虽然许多物质破坏生理胃粘膜屏障，很少有人知道他们的超微结构后遗症。我们选择了两种不同的屏障破坏剂-乙醇和尿素进行形态学研究。禁食Swiss小鼠通过食管插管接受乙醇、尿素或对照溶液，持续时间长达15分钟。乙醇和尿素产生的形态学变化，在一些动物早在2分钟的曝光后。乙醇的第一个可观察到的影响涉及表面粘液细胞内的细胞核和细胞质的变化，而没有明显的顶端细胞膜或紧密连接的破坏。我们从这些发现中推断，乙醇的脂溶性可能使其能够通过顶端膜或紧密连接迅速扩散，而不会造成明显的损伤，直到它在细胞内。与此相反，尿素溶液最初引起的表面粘液细胞内的紧密连接和空泡的形成扭曲，而其余的细胞似乎正常。这些观察结果表明，尿素，相对不渗透穿过胃上皮，可能会导致损伤通过渗透力。这些研究表明，虽然不同的胃粘膜刺激物产生相似的生理效应，但它们在损伤的形态学顺序上并不一致。
Although many substances break the physiological gastric mucosal barrier, little is known regarding their ultrastructural sequelae. We selected for morphological study two dissimilar barrier breakers—ethanol and urea. Fasted Swiss mice received, via esophageal intubation, ethanol, urea, or control solutions for periods up to 15 min. Both ethanol and urea produced morphological changes in some animals as early as after 2-min exposure. The first observable effects of ethanol involved nuclear and cytoplasmic changes within the surface mucous cell without apparent disruption of the apical cell membrane or tight junction. We infer from these findings that the lipid solubility of ethanol may allow it to diffuse rapidly through the apical membrane or tight junction without causing appreciable damage until it is within the cell. In contrast, urea solutions initially caused distortion of the tight junctions and formation of vacuoles within the surface mucous cell, while the remainder of the cell appeared normal. These observations suggest that urea, being relatively impermeant across gastric epithelium, may cause injury via osmotic forces. These studies indicate that, although different gastric mucosal irritants produce similar physiological effects, they are not uniform in their morphological sequence of injury.