Perilipin 1-3in grass carpCtenopharyngodon idella: molecular characterization, gene structure, tissue distribution, and mRNA expression in DHA-induced lipid droplet formation in adipocytes
Perilipin 1-3in grass carpCtenopharyngodon idella: molecular characterization, gene structure, tissue distribution, and mRNA expression in DHA-induced lipid droplet formation in adipocytes
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草鱼 Perilipin 1-3 DHA 诱导脂肪细胞脂滴形成的分子特征、基因结构、组织分布和 mRNA 表达
DOI：
10.1007/s10695-020-00857-x
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发表时间：
2020-09-30
影响因子：
2.9
通讯作者：
Ji, Hong
中科院分区：
文献类型：
--
作者：
Huang, Xiaocheng;Sun, Jian;Ji, Hong
Perilipin家族是细胞内中性脂质储存池脂滴(LD)的主要结构蛋白，调节LD的组装和形成，对脂质代谢至关重要。本文从草鱼中鉴定了 perilipin 家族的三个旁系同源物，并获得了它们的完整编码序列（CDS），包括 perilipin1、perilipin2 和 perilipin3，分别编码 492、454 和 419 个氨基酸的肽。草鱼周脂蛋白推导的氨基酸序列与其他硬骨鱼物种的同源性比对表明，与哺乳动物的同源性低于55%。哺乳动物中的 PAT (perilipin) 结构域也在草鱼 perilipin 1-3 蛋白中被预测。基因组组织分析显示，草鱼perilipin1包含6个编码外显子，而perilipin2和perilipin3均包含7个编码外显子。编码三种旁系同源物的 mRNA 在多种组织中表达； perilipin1-3主要表达于脂肪组织和肝脏；此外，perilipin3在心脏中也高表达。在体外，200μM DHA有效增加草鱼完全分化脂肪细胞中较小脂滴的比例。 DHA处理的脂肪细胞中perilipin1、perilipin2和perilipin3 mRNA表达显着增加（P < 0.05，P < 0.01）。 DHA 处理期间脂肪细胞中不同旁系同源物的相同反应表明它们可能在 LD 的形成中发挥协同作用。
Perilipin family is the main structural proteins of lipid droplet (LD) that is intracellular neutral lipid store ponds, and regulates LD assembly and formation, and is crucial for lipid metabolism. Here three paralogs of perilipin family were characterized from grass carp and their complete coding sequences (CDS) were obtained, including perilipin1, perilipin2, and perilipin3, coding peptides of 492, 454, and 419 amino acids, respectively. The alignment of the homology of grass carp perilipin deduced amino acid sequences with other teleost species showed that the homology with mammalian was less than 55%. PAT (perilipin) domain in mammalian was also predicted in grass carp perilipin 1-3 proteins. Genomic organization analysis revealed that grass carp perilipin1 contained 6 coding exons, while both perilipin2 and perilipin3 consisted of 7 coding exons. The mRNA encoding three paralogs were expressed in a wide range of tissues; perilipin1-3 were primarily expressed in adipose tissue and liver; besides, perilipin3 was also highly expressed in the heart. In vitro, 200 mu M DHA increased the proportion of smaller lipid droplets effectively in fully differentiated adipocytes of grass carp. The mRNA expression of perilipin1, perilipin2, and perilipin3 was significantly increased in the adipocytes treated with DHA (P < 0.05,P < 0.01). The same responses of different paralogs in the adipocytes during DHA treatment suggest that they might play synergistic roles in the formation of LDs.