Translation initiation with 70S ribosomes:: an alternative pathway for leaderless mRNAs
Translation initiation with 70S ribosomes:: an alternative pathway for leaderless mRNAs
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DOI：
10.1093/nar/gkh663
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发表时间：
2004-06-01
影响因子：
14.9
通讯作者：
Bläsi, U
中科院分区：
文献类型：
--
作者：
Moll, I;Hirokawa, G;Bläsi, U
一般认为细菌中的翻译是由30S核糖体亚基启动的。相比之下，几条相当间接的体外证据表明，70 S单体能够启动无前导mRNA的翻译，起始密码子的A开始。在这项研究中，我们证明了专用70 S核糖体在体外翻译无前导mRNA的能力。在支持，我们表明，一个自然的领导mRNA可以翻译与交联的70 S野生型核糖体。此外，我们报告说，leaderless mRNA翻译继续在70S核糖体的患病率在体内创建的条件下，并在批量mRNA的翻译停止。这些研究提供了在体内以及直接在体外的证据为70 S起始途径的天然存在的leaderless mRNA，并讨论了在不利条件下的细菌生长的意义和它们的进化意义的翻译。
It is generally accepted that translation in bacteria is initiated by 30S ribosomal subunits. In contrast, several lines of rather indirect in vitro evidence suggest that 70S monosomes are capable of initiating translation of leaderless mRNAs, starting with the A of the initiation codon. In this study, we demonstrate the proficiency of dedicated 70S ribosomes in in vitro translation of leaderless mRNAs. In support, we show that a natural leaderless mRNA can be translated with crosslinked 70S wild-type ribosomes. Moreover, we report that leaderless mRNA translation continues under conditions where the prevalence of 70S ribosomes is created in vivo, and where translation of bulk mRNA ceases. These studies provide in vivo as well as direct in vitro evidence for a 70S initiation pathway of a naturally occurring leaderless mRNA, and are discussed in light of their significance for bacterial growth under adverse conditions and their evolutionary implications for translation.