DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES .I. GROWTH AT LOW TEMPERATURES
DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES .I. GROWTH AT LOW TEMPERATURES
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DOI：
10.1128/jb.94.1.27-31.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
HARRIS, VG
中科院分区：
文献类型：
--
作者：
JOHNSON, RC;HARRIS, VG
致病性钩端螺旋体的最低生长温度为13 - 15 ℃。嗜血钩端螺旋体的最低生长温度为5 - 10 ℃，或比病原体低约5 ℃。嗜血钩端螺旋体在低于致病钩端螺旋体生长温度下的生长能力提供了一种简单的鉴别方法。当接种物在试验培养基中产生约8 × 107个细胞/ml，孵育温度为13 ℃时，嗜血性钩端螺旋体很容易与致病性钩端螺旋体区分开来。所有13个被测的嗜血钩端螺旋体在13 ℃的10%兔血清培养基中生长，而20个病原体在30天的潜伏期内没有一个生长。
The minimal growth temperature of the pathogenic leptospires is between 13 and 15 C. The saprophytic leptospires have a minimal growth temperature between 5 and 10 C, or approximately 5 C below that of the pathogens. The capability of the saprophytic leptospires to grow at temperatures below those which allow the growth of the pathogenic leptospires provides a simple method of discrimination. With an inoculum yielding approximately 8 X 107 cells per ml in the test medium and an incubation temperature of 13 C, the saprophytic leptospires were easily differentiated from the pathogenic leptospires. All 13 saprophytic leptospires tested grew in the 10 percent rabbit serum medium at 13 C, whereas none of the 20 pathogens grew during the 30-day incubation period.