Engineering of self-sustaining systems: Substituting the yeast glucose transporter plus hexokinase for the Lactococcus lactis phosphotransferase system in a Lactococcus lactis network in silico
Engineering of self-sustaining systems: Substituting the yeast glucose transporter plus hexokinase for the Lactococcus lactis phosphotransferase system in a Lactococcus lactis network in silico
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DOI：
10.1002/biot.201100314
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发表时间：
2012-07-01
影响因子：
4.7
通讯作者：
Westerhoff, Hans V.
中科院分区：
文献类型：
--
作者：
Adamczyk, Malgorzata;Westerhoff, Hans V.
将新功能引入一个生物物种的成功率仍然相当不令人满意。这在很大程度上是由于活态的本质，即它对扰动的鲁棒性。活细胞一定会注意到代谢工程正在通过代谢物浓度的变化而受到影响。在这项研究中，我们询问是否可以在不改变代谢物浓度的情况下进行这种工程。我们已经说明了，在硅片上，原则上可以这样做。我们已经在用酵母葡萄糖转运蛋白加己糖激酶代替乳酸乳球菌磷酸转移酶系统的情况下，在一个L。lactis网络，这种工程在代谢物浓度和几乎所有通量方面是“沉默的”。
The success rate of introducing new functions into a living species is still rather unsatisfactory. Much of this is due to the very essence of the living state, i.e. its robustness towards perturbations. Living cells are bound to notice that metabolic engineering is being effected, through changes in metabolite concentrations. In this study, we asked whether one could engage in such engineering without changing metabolite concentrations. We have illustrated that, in silico, one can do so in principle. We have done this for the case of substituting the yeast glucose transporter plus hexokinase for the Lactococcus lactis phosphotransferase system, in an L. lactis network, this engineering is 'silent' in terms of metabolite concentrations and almost all fluxes.