High resolution SIMS analysis of arsenic in rice
High resolution SIMS analysis of arsenic in rice
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DOI：
10.1002/sia.4903
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发表时间：
2013-01
影响因子：
1.7
通讯作者：
K. Moore;C. Hawes;S. McGrath;F. Zhao;C. Grovenor
中科院分区：
文献类型：
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作者：
K. Moore;C. Hawes;S. McGrath;F. Zhao;C. Grovenor
以亚细胞分辨率确定生物材料中微量元素的分布非常具有挑战性，但对于了解其吸收机制至关重要。稻米中砷 (As) 的积累非常有效，可能对东南亚数百万人构成严重的健康风险。 NanoSIMS 是一款最先进的显微镜，能够进行高分辨率化学成像（低至 50 nm）并检测极低的元素浓度（百万分之一的水平）。这使其非常适合生物材料中的微量元素定位。本文展示了如何使用 NanoSIMS 以高分辨率研究稻谷中砷的定位，以及不同的处理如何导致稻谷中的不同分布。还显示了水稻根部亚细胞尺度上百万分之一砷浓度的精确位置及其与其他元素的定位。版权所有 © 2012 约翰·威利父子有限公司
Determining the distribution of trace elements in biological materials with subcellular resolution is very challenging but vitally important in order to understand their mechanisms of uptake. Rice grain is efficient in the accumulation of arsenic (As) in the grain, potentially posing a severe health risk to millions of people in South‐East Asia. The NanoSIMS is a state‐of‐the‐art microscope capable of high resolution chemical imaging (down to 50 nm) and detecting very low elemental concentrations (parts‐per‐million levels). This makes it ideally suited for trace element localisation in biological materials. This paper shows how the NanoSIMS can be used to investigate the localisation of As in rice grain at high resolution and how different treatments result in different distributions in the grain. The precise location of parts‐per‐million As concentrations and its localisation with other elements at the subcellular scale in rice roots are also shown. Copyright © 2012 John Wiley & Sons, Ltd.