Plasmonic photothermal destruction of uropathogenic E. coli with reduced graphene oxide and core/shell nanocomposites of gold nanorods/reduced graphene oxide.
Plasmonic photothermal destruction of uropathogenic E. coli with reduced graphene oxide and core/shell nanocomposites of gold nanorods/reduced graphene oxide.
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DOI：
10.1039/c4tb01760a
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发表时间：
2015-01
期刊：
影响因子：
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通讯作者：
K. Turcheniuk;Charles-Henri Hage;J. Spadavecchia;Aritz Yanguas Serrano;Iban Larroulet;A. Pesquera;A. Zur
中科院分区：
文献类型：
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作者：
K. Turcheniuk;Charles-Henri Hage;J. Spadavecchia;Aritz Yanguas Serrano;Iban Larroulet;A. Pesquera;A. Zur
开发针对革兰氏阴性尿路致病性大肠杆菌细菌感染的非抗生素疗法是一项复杂的任务。因此迫切需要治疗此类感染的新策略。本报告阐述了聚乙二醇化还原氧化石墨烯纳米颗粒 (rGO-PEG) 和涂有 rGO-PEG (rGO-PEG-Au NR) 的金纳米棒 (Au NR) 的开发，用于选择性杀死尿路致病性大肠杆菌 UTI89。我们利用 rGO-PEG 和 Au NR 颗粒在近红外 (NIR) 区域的优异光吸收特性，通过照射含有细菌的溶液，在 10 分钟内光热杀死革兰氏阴性病原体高达 99%。 rGO-PEG-Au NRs 对大肠杆菌表现出比 rGO-PEG 更好的光热效率。使用多聚庚基 α-d-甘露糖苷探针功能化的 rGO-PEG-Au NR 可以实现对大肠杆菌 UTI89 的靶向杀灭。目前，这为消除病原体提供了一种独特的生物相容性方法，为尿路感染患者的临床治疗开辟了新的可能性。
The development of non-antibiotic based treatments against bacterial infections by Gram-negative uropathogenic E. coli is a complex task. New strategies to treat such infections are thus urgently needed. This report illustrates the development of pegylated reduced graphene oxide nanoparticles (rGO-PEG) and gold nanorods (Au NRs) coated with rGO-PEG (rGO-PEG-Au NRs) for the selective killing of uropathogenic E. coli UTI89. We took advantage of the excellent light absorption properties of rGO-PEG and Au NR particles in the near-infrared (NIR) region to photothermally kill Gram-negative pathogens up to 99% in 10 min by illumination of solutions containing the bacteria. The rGO-PEG-Au NRs demonstrated better photothermal efficiency towards E. coli than rGO-PEG. Targeted killing of E. coli UTI89 could be achieved with rGO-PEG-Au NRs functionalized with multimeric heptyl α-d-mannoside probes. This currently offers a unique biocompatible method for the ablation of pathogens with the opening of probably a new possibility for clinical treatments of patients with urinary infections.