Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin
Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin
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DOI：
10.1006/geno.1996.4493
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发表时间：
1997-02-01
期刊：
影响因子：
4.4
通讯作者：
Honjo, T
中科院分区：
文献类型：
--
作者：
Kurooka, H;Kato, K;Honjo, T
在接近小鼠11号染色体裸位点的酵母人工染色体重叠群中，我们发现了一个新的基因，核氧还蛋白，它编码一种与硫氧还蛋白活性位点相似的蛋白。核氧还蛋白基因在哺乳动物中是保守的，仅在秀丽隐杆线虫中发现了两个同源基因。核氧还蛋白转录物在所有检查的成体组织中表达，但限于第10.5-11.5天胚胎的神经系统和肢芽。核氧还蛋白蛋白主要定位于用核氧还蛋白表达构建体转染的细胞的细胞核中，由于核氧还蛋白的细菌表达蛋白具有氧化还原性，由于硫氧还蛋白具有胰岛素二硫键的还原酶活性，其动力学与硫氧还蛋白相似，因此它可能是核蛋白如转录因子的氧化还原调节剂。(C)北京：科学出版社.
In a yeast artificial chromosome contig close to the nude locus on mouse chromosome 11, we identified a novel gene, nucleoredoxin, that encodes a protein with similarity to the active site of thioredoxins. Nucleoredoxin is conserved between mammalian species, and two homologous genes mere found in Caenorhabditis elegans. The nucleoredoxin transcripts are expressed in all adult tissues examined, but restricted to the nervous system and the limb buds in Day 10.5-11.5 embryos, The nucleoredoxin protein is predominantly localized in the nucleus of cells transfected with the nucleoredoxin expression construct, Since the bacterially expressed protein of nucleoredoxin showed oxido-reductase activity of the insulin disulfide bonds with kinetics similar to that of thioredoxin, it may be a redox regulator of the nuclear proteins, such as transcription factors. (C) 1997 Academic Press.