Measuring the impacts of Roundup Original® on fluctuating asymmetry and mortality in a Neotropical tadpole
Measuring the impacts of Roundup Original® on fluctuating asymmetry and mortality in a Neotropical tadpole
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DOI：
10.1007/s10750-015-2404-0
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发表时间：
2016-02-01
期刊：
影响因子：
2.6
通讯作者：
Nomura, Fausto
中科院分区：
文献类型：
--
作者：
Costa, Renan Nunes;Nomura, Fausto
两栖类幼虫对污染非常敏感，这可能导致致命和亚致命的影响。这种影响可以通过波动不对称性（FA）来衡量，这是基于生物体两侧对称性之间的差异。我们评估了急性和慢性暴露于Roundup Original（R）对红酸浆属（Physalaemus cuvieri）蝌蚪的影响。我们测量了蝌蚪的存活率并估计了LC 50（96 h）。我们还评估了亚致死浓度是否会增加FA。在急性接触中，存活率降低，LC 50为2.13 mg a.i./ L.在慢性暴露中，鼻距和眼宽有显着较高的FA污染蝌蚪。长期接触污染物可能导致若干亚致死效应，这些效应将用于生物监测调查。受污染物影响的形态特征，如畸形或FA，将相对更容易从田间样品中测量。因为它具有成本效益，易于测量，并且可以在不标记或饲养野外捕获的动物的情况下获得，我们建议FA是一种有前途的标记物，用于监测农达等污染物对环境的影响。然而，有必要进行更多的研究，以了解哪些额外的环境压力可能会影响FA，以及这可能会如何改变其在生物监测中的用途。
Amphibian larvae are highly susceptible to contamination, which can lead to lethal and sublethal effects. This impact can be measured by fluctuating asymmetry (FA), which is based on differences between the sides of organisms with bilateral symmetry. We evaluated the effect of acute and chronic exposure to Roundup Original ((R)) on Physalaemus cuvieri tadpoles. We measured tadpole survival and estimated the LC50(96h). We also evaluated whether a sublethal concentration increases the FA. In acute exposure, survival was reduced and the LC50 was 2.13 mg a.i./l. In chronic exposure, nostril-snout distance and eye width had a significantly higher FA in contaminated tadpoles. The chronic exposure to contaminants could lead to several sublethal effects, which would be used in biomonitoring surveys. Morphological traits affected by contaminants, such as malformations or FA, would be relatively more easily measured from field samples. Because it is cost effective, easy to measure, and can be obtained without tagging or housing field-caught animals, we suggest that FA is a promising marker for monitoring the environmental impacts of contaminants like Roundup. However, additional studies are necessary to understand what additional environmental stressors might impact FA, and how this might alter its utility for use in biomonitoring.