In vitro and in vivo expression of Gal (cid:1) –(1,3)Gal on porcine islet cells is age dependent
In vitro and in vivo expression of Gal (cid:1) –(1,3)Gal on porcine islet cells is age dependent
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猪胰岛细胞上 Gal (cid:1) –(1,3)Gal 的体外和体内表达具有年龄依赖性
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发表时间：
2003
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通讯作者：
G. Korbutt
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作者：
G. Rayat;R. Rajotte;B. Hering;T. Binette;G. Korbutt
Gal(Cid：1)-(1，3)Gal((Cid：1)Gal)在猪胰岛细胞上的表达仍存在争议。几个小组已经报道了猪胰岛内分泌细胞不表达(Cid：1)Gal，而我们在新生猪胰岛(NPI)中发现(Cid：2)细胞确实表达这种抗原。我们假设在NPI上表达(Cid：1)Gal的内分泌细胞是不太成熟的细胞，可能起源于导管细胞，当他们发育成完全成熟的(Cid：2)细胞时，这种抗原的表达消失。因此，我们进一步检测了(CID：1)Gal在不同猪胰岛细胞制剂上的表达，并将其与细胞角蛋白7(CK7)阳性的导管细胞的比例相关联。在体外和体内，(Cid：1)Gal和CK7在未成熟的非成熟猪胰岛细胞中的表达显著高于成熟的非成熟猪胰岛细胞和成年猪胰岛细胞(P<；0.05)，而(Cid：2)细胞的表达比例则相反(P&fi；0.05)。此外，在表达Gal的细胞中，fi阳性细胞的比例明显高于不表达的细胞。相反，与Gal阳性人群相比，Gal阴性人群中观察到的(Cid：2)细胞比例更高。这些数据表明，随着猪胰岛细胞成熟为(CID：2)细胞，(CID：1)Gal和CK7的表达减少，这表明(Cid：1)Gal可能在胰腺内分泌(CID：2)细胞的成熟中起作用。
The expression of Gal (cid:1) –(1,3)Gal ( (cid:1) Gal) on porcine islet cells remains controversial. Several groups have reported that porcine islet endocrine cells do not express (cid:1) Gal while we have shown in neonatal porcine islets (NPI) that (cid:2) cells do express this antigen. We hypothesize that endocrine cells expressing (cid:1) Gal on NPI are less mature cells that may have originated from ductal cells and that expression of this antigen disappears as they develop into fully mature (cid:2) cells. Thus, we further examined (cid:1) Gal expression on various porcine islet cell preparations and correlated this with the proportion of cytokeratin 7 (CK7)-positive ductal cells. In vitro and in vivo expression of (cid:1) Gal and CK7 was significantly ( P < 0·05) higher in less mature NPI cells compared with matured NPI and adult porcine islet cells while the reverse was observed in the proportion of (cid:2) cells. Moreover, a significantly higher proportion of CK7-positive cells was detected in the Gal-expressing population compared with non-expressing cells. In contrast, a higher proportion of (cid:2) cells was observed in the Gal-negative population compared with the Gal-positive population. These data showed a reduced expression of (cid:1) Gal and CK7 as porcine islet cells mature into (cid:2) cells suggesting a possible role for (cid:1) Gal in the maturation of pancreatic endocrine (cid:2) cells.