A conserved unusual posttranscriptional processing mediated by short, direct repeated (SDR) sequences in plants
A conserved unusual posttranscriptional processing mediated by short, direct repeated (SDR) sequences in plants
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植物中由短直接重复（SDR）序列介导的保守的不寻常转录后加工
DOI：
10.1016/s1673-8527(09)60028-x
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发表时间：
2010-01-01
影响因子：
5.9
通讯作者：
Liu, Yongsheng
中科院分区：
文献类型：
--
作者：
Niu, Xiangli;Luo, Di;Liu, Yongsheng
在单子叶谷类作物的几个逆境反应基因座中，我们已经发现了一种不寻常的转录后加工，它是由5‘和3’剪接接头上的短直接重复序列(SDR)的配对存在所介导的，这些剪接接头不同于传统的(U2/U12型)剪接边界。利用已知的含有SDR的序列作为探针，在GenBank数据库中预测了24个植物候选基因，这些基因参与了单子叶和双子叶植物不同的功能途径，可能具有SDR介导的转录后加工。SDRs介导的转录后加工事件，包括顺式和反式作用，在大多数预测的候选基因中被实验检测到。广泛的序列分析显示了几种与SDR相关的剪接特性，包括部分外显子缺失、外显子片段重复、外显子片段扰乱和反式剪接，导致部分外显子丢失或RNA分子内部或之间异常的外显子序列重排。此外，我们还证明了在瞬时表达和稳定转化系统中，SDR的配对存在是必要的，但不是充分的。我们还发现原核生物不能进行SDR介导的前mRNA剪接。
In several stress responsive gene loci of monocot cereal crops, we have previously identified an unusual posttranscriptional processing mediated by paired presence of short direct repeated (SDR) sequences at 5' and 3' splicing junctions that are distinct from conventional (U2/U12-type) splicing boundaries. By using the known SDR-containing sequences as probes, 24 plant candidate genes involved in diverse functional pathways from both monocots and dicots that potentially possess SDR-mediated posttranscriptional processing were predicted in the GenBank database. The SDRs-mediated posttranscriptional processing events including cis- and trans-actions were experimentally detected in majority of the predicted candidates. Extensive sequence analysis demonstrates several types of SDR-associated splicing peculiarities including partial exon deletion, exon fragment repetition, exon fragment scrambling and trans-splicing that result in either loss of partial exon or unusual exonic sequence rearrangements within or between RNA molecules. In addition, we show that the paired presence of SDR is necessary but not sufficient in SDR-mediated splicing in transient expression and stable transformation systems. We also show prokaryote is incapable of SDR-mediated premRNA splicing.