THE UL13 GENE OF HERPES-SIMPLEX VIRUS-1 ENCODES THE FUNCTIONS FOR POSTTRANSLATIONAL PROCESSING ASSOCIATED WITH PHOSPHORYLATION OF THE REGULATORY PROTEIN-ALPHA-22
THE UL13 GENE OF HERPES-SIMPLEX VIRUS-1 ENCODES THE FUNCTIONS FOR POSTTRANSLATIONAL PROCESSING ASSOCIATED WITH PHOSPHORYLATION OF THE REGULATORY PROTEIN-ALPHA-22
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DOI：
10.1073/pnas.89.16.7310
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发表时间：
1992-08-15
影响因子：
11.1
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
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作者：
PURVES, FC;ROIZMAN, B
单纯疱疹病毒 1 基因组显示编码两个基因 U(S)3 和 U(L)13，表现出蛋白激酶共有的氨基酸序列基序。该实验室在其他地方报告说，U(S)3 蛋白激酶的主要底物是 U(L)34 基因的产物，U(L)34 基因是一种必需的非糖基化膜蛋白。在没有 U(S)3 激酶的情况下，U(L)34 蛋白保持未磷酸化状态，但与四种蛋白形成复合物，当 U(L)34 不磷酸化时，这四种蛋白会独特地被磷酸化。为了研究U(L)13蛋白在此过程中的作用，构建了缺乏U(L)13或U(L)13和U(S)3两者的重组病毒。我们报告说，U(L)13 对于细胞培养中的病毒复制是可有可无的，并且不参与 U(L)34 或相关磷蛋白的加工。然而，U(L)13 负责与受感染细胞蛋白 22（α-22 基因的产物）磷酸化相关的翻译后加工。此前曾报道该基因在选定的细胞系中发挥调节作用。 U(L)13 似乎是蛋白激酶或磷酸转移酶，其主要底物是 alpha-22 蛋白。
The herpes simplex virus 1 genome was shown to encode two genes, U(S)3 and U(L)13, exhibiting amino acid sequence motifs common to protein kinases. Elsewhere this laboratory reported that the prominent substrate of the U(S)3 protein kinase is the product of the U(L)34 gene, an essential nonglycosylated membrane protein. In the absence of the U(S)3 kinase, the U(L)34 protein remains unphosphorylated but forms a complex with four proteins that become phosphorylated uniquely when U(L)34 is not. To investigate the role of U(L)13 protein in this process, recombinant viruses lacking U(L)13 or both U(L)13 and U(S)3 were constructed. We report that U(L)13 is dispensable for viral replication in cell culture and is not involved in the processing of U(L)34 or of associated phosphoproteins. U(L)13 is, however, responsible for the posttranslational processing associated with phosphorylation of infected-cell protein 22, the product of the alpha-22 gene. This gene was previously reported to play a regulatory role in selected cell lines. U(L)13 appears to be either a protein kinase or a phosphotransferase and its major substrate is the alpha-22 protein.