Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance
Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance
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DOI：
10.1248/bpb.28.1505
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发表时间：
2005-08-01
影响因子：
2
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
Ogawa, W;Li, DW;Tsuchiya, T
临床分离的肺炎克雷伯菌MGH78578对多种抗菌药物表现出较高的耐药性。我们从克雷伯氏菌的染色体DNA中克隆了耐药基因。pneumoniae MGH78578，采用鸟枪法，以药物过敏菌株Escherichia coli KAM 32为宿主。我们获得了43个杂合质粒，使宿主细胞对几种抗菌药物耐药。我们将它们分为17组的基础上，在每一个9种抗菌剂的存在下，每个混合质粒的限制性模式的生长特性。克隆基因的分析对于研究克雷伯菌多药耐药系统的主要部分包括多药外排泵具有重要意义。pneumoniae MGH78578，其中基因组序列是可获得的。
Klebsiella pneumoniae MGH78578, a clinical isolate, showed high level of resistance to many antimicrobial agents. We cloned genes responsible for drug resistance from chromosomal DNA of K. pneumoniae MGH78578 by shotgun method using Escherichia coli KAM32, a drug hypersensitive strain, as host. We obtained 43 hybrid plasmids that made host cells resistant to several antimicrobial agents. We classified them into 17 groups based on growth properties in the presence of each one of 9 antimicrobial agents and on restriction patterns of each hybrid plasmid. Analysis of the cloned genes must be very useful for investigation of major parts of multidrug resistance systems including multidrug efflux pumps in K. pneumoniae MGH78578 in which genome sequence is available.