MOLECULAR-CLONING OF THE INTERLEUKIN-1-BETA CONVERTING ENZYME
MOLECULAR-CLONING OF THE INTERLEUKIN-1-BETA CONVERTING ENZYME
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DOI：
10.1126/science.1373520
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发表时间：
1992-04-03
期刊：
影响因子：
56.9
通讯作者：
BLACK, RA
中科院分区：
文献类型：
--
作者：
CERRETTI, DP;KOZLOSKY, CJ;BLACK, RA
白细胞介素-1- β (il -1- β)介导广泛的免疫和炎症反应。活性细胞因子是由无活性前体的蛋白水解裂解产生的。一个编码进行这种切割的蛋白酶的互补DNA已经被克隆出来。重组表达在COS-7细胞中，使细胞能够将前体il -1- β加工成成熟的形式。序列分析表明，该酶本身可能经历了蛋白水解过程。编码蛋白酶的基因被定位到染色体带11q23，这是一个经常涉及人类癌症重排的位点。
Interleukin-1-beta (IL-1-beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1-beta to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.