Axenic Cultivation of the Dioecious Nematode Panagrellus Redivivus
Axenic Cultivation of the Dioecious Nematode Panagrellus Redivivus
复制标题
雌雄异体线虫 Panagrellus Redivivus 的无菌培养
DOI：
10.1163/187529263x00818
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
E. Yarwood
中科院分区：
文献类型：
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作者：
W. S. Cryan;E. Hansen;M. Martín;F. W. Sayre;E. Yarwood
将自由生活的线虫Panagrellus redivus在连续无菌培养中保持了6个月。在使用抗菌剂的半自动装置中，将P. redivus从细菌中清除。已在含有大肠杆菌的营养琼脂斜面上建立并维持了单菌培养，在含有大豆蛋白胨、酵母提取物和加热的肝提取物的液体培养基中建立并维持了无菌培养。通过在补充有纯化蛋白生长因子（40 μg/ml）的化学成分确定的培养基中进行连续传代培养，维持了P. redivivus的培养物，该培养基在为用雄性先熟雌雄同体，Cajorhabditis briggsae（小杆线虫科）进行营养研究而设计的规定条件下进行。P. redivivus似乎是专性雌雄异株。在液体培养基中培养不会损害繁殖。与C. Briggsae，P. redivivus在4° C下能存活3个月。
The free living nematode Panagrellus redivivus has been maintained in serial axenic culture for 6 months. P. redivivus was freed from bacteria in a semi-automatic device using antimicrobial agents. Cultures have been established and maintained monoxenically on nutrient agar slants with Escherichia coli, and axenically in a liquid medium containing soya peptone, yeast extract and heated liver extract. Cultures of P. redivivus have been maintained by serial subcultures in a chemically defined medium supplemented with purified protein growth factor (40 μg/ml) under the defined conditions designed for nutritional studies with the protandrous hermaphrodite, Caenorhabditis briggsae (Rhabditidae) . P. redivivus appears to be obligately dioecious. Reproduction has not been impaired by cultivation in liquid media. In marked contrast to C. briggsae, P. redivivus is able to survive 4° C for 3 months.