TISSUE CONCENTRATIONS OF SOMATOMEDIN-C - FURTHER EVIDENCE FOR MULTIPLE SITES OF SYNTHESIS AND PARACRINE OR AUTOCRINE MECHANISMS OF ACTION
TISSUE CONCENTRATIONS OF SOMATOMEDIN-C - FURTHER EVIDENCE FOR MULTIPLE SITES OF SYNTHESIS AND PARACRINE OR AUTOCRINE MECHANISMS OF ACTION
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DOI：
10.1073/pnas.81.3.935
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
UNDERWOOD, LE
中科院分区：
文献类型：
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作者：
DERCOLE, AJ;STILES, AD;UNDERWOOD, LE
验证了一种用于提取和测量生长调节素C（Sm-C）/胰岛素样生长因子I（IGF-I）（一种生长激素依赖性生长促进肽）组织含量的方法。Sm-C含量的组织提取物是强烈的生长激素依赖性，因为大多数的组织研究垂体切除大鼠含有显着低于Sm-C比正常组织。对垂体切除大鼠腹腔注射绵羊生长激素（oGH）导致肾脏、肝脏、肺、心脏和睾丸中组织可提取Sm-C增加。组织Sm-C对oGH的反应在12 h后达到最大，在血清中最大增量之前6 h。在肝脏和肺中，组织Sm-C对不同剂量oGH的反应符合线性回归模型，增加Sm-C所需的oGH剂量在增加蛋白质合成所需的范围内。虽然这些结果并不排除生长调节素的作用通过类内分泌机制的可能性，但它们增加了对这些肽通过自分泌或旁分泌机制起作用的概念的支持，这些肽在多个位点产生并在其产生位点或附近起作用。
A method was validated for extracting and measuring the tissue content of somatomedin C (Sm-C)/insulin-like growth factor I (IGF-I), a growth-hormone-dependent, growth-promoting peptide. The Sm-C content of tissue extracts was strongly growth-hormone dependent, because most of the tissues studied from hypophysectomized rats contained significantly less Sm-C than normal tissues. The i.p. administration of ovine growth hormone (oGH) to hypophysectomized rats caused tissue extractable Sm-C to increase in kidney, liver, lung, heart, and testes. Tissue Sm-C responses to oGH were maximal after 12 h, 6 h before the maximal increment in serum. In liver and lung, the tissue Sm-C response to various doses of oGH fit linear regression models, and the doses of oGH needed to increase the Sm-C are in the range of those required to increase protein synthesis. Although these results do not exclude the possibility that the somatomedins act by hormone-like endocrine mechanisms, they add support to the concept that these peptides act through autocrine or paracrine mechanisms, being produced at multiple sites and acting at or near their sites of production.