Inhibition of Tomato Early Blight Disease by Culture Extracts of a Streptomyces puniceus Isolate from Mangrove Soil
Inhibition of Tomato Early Blight Disease by Culture Extracts of a Streptomyces puniceus Isolate from Mangrove Soil
复制标题
红树林土壤中的红树链霉菌培养提取物对番茄早疫病的抑制作用
DOI：
10.1094/phyto-12-18-0444-r
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发表时间：
2019-07-01
期刊：
影响因子：
3.2
通讯作者：
Xu, Ying
中科院分区：
文献类型：
--
作者：
Hao, Lingyun;Zheng, Xiaoli;Xu, Ying
本研究探讨了来自红树林根际细菌的天然产物在番茄早疫病防治中的潜力。从香港米埔自然保护区老鼠仓（Acanthus ilicifolius Linn）根际分离到一株紫红链霉菌（Streptomyces puniceus）L75。L75发酵液乙酸乙酯粗提物具有广谱抗真菌活性。与代森锰锌相比，L75 EA提取物在25 μ g/ml或更低的浓度下对番茄早疫病菌生长抑制显著更有效，对番茄叶或根发育没有可观察到的负面影响。在相同浓度下，L75 EA提取物的水生毒性显著低于代森锰锌。L75 EA提取物靶向A. Solani分生孢子，具有抑真菌作用模式。液相色谱-四极杆飞行时间质谱分析鉴定了两种可能的抗真菌化合物，Alteramide A和热稳定抗真菌因子，它们共同对L75 EA提取物的生物活性起部分作用。在离体番茄叶片上，接种A. 5天后，相对于对照，具有50、25或5 μ g/ml的L75 EA提取物的茄属植物分别使患病面积减少约98%、约90%和约48%。这项研究表明了红树林根际细菌天然产物在农业应用中的潜力。
This study investigates the potential of natural products derived from a mangrove rhizosphere bacterium in tomato early blight management. A Streptomyces puniceus strain L75 was isolated from the rhizosphere of Acanthus ilicifolius Linn in the Mai Po Reserve, Hong Kong. The crude ethyl acetate (EA) extract of L75 fermentation cultures has broadspectrum antifungal bioactivities. L75 EA extract was significantly more effective in Alternaria solani growth inhibition at 25 mu g/ml or lower compared with Mancozeb, with no observable negative impacts on tomato leaves or root development. Furthermore, L75 EA extract had significantly lower aquatic toxicity than Mancozeb at the same concentrations. L75 EA extract targets germ tube elongation of A. solani conidia, with a fungistatic mode of action. Liquid chromatographyquadrupole time-of-flight mass spectrometry analysis identified two possible antifungal compounds, Alteramide A and the Heat-Stable Antifungal Factor, which together contribute partially to the bioactivity of L75 EA extract. On detached tomato leaves, coinoculation of A. solani with L75 EA extract of 50, 25, or 5 mu g/ml reduced diseased areas by similar to 98, similar to 90, and similar to 48%, respectively, relative to the control after 5 days. This study demonstrates the potential of natural products from mangrove rhizosphere bacteria in agricultural applications.