An automatic diffraction data collection system with an imaging plate

An automatic diffraction data collection system with an imaging plate
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一种带有成像板的自动衍射数据采集系统

DOI:
10.1107/s0021889890004009
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发表时间:
1990
影响因子:
6.1
通讯作者:
C. Katayama
C. Katayama
中科院分区:
材料科学3区
文献类型:
--
作者:
I. Tanaka;Mingde Yao;Mamoru Suzuki;K. Hikichi;T. Matsumoto;M. Kozasa;C. Katayama

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已开发出用于蛋白质晶体学的带有成像板的自动衍射数据收集系统。该系统的工作方式与传统的旋转相机方法类似,但处于完全在线模式。暴露于 X 射线束后,成像板(半径为 100 mm)旋转,同时读取头扫描成像板,以类似于电唱机的方式测量受激发光。在下一个曝光期间,并行处理之前拍摄的图像。该系统已经使用肽和蛋白质晶体进行了测试,并被证明可以成功工作。
An automatic diffraction data collection system with an imaging plate has been developed for protein crystallography. The system works in a similar way to the conventional rotation camera method, but in full online mode. After exposure to the X-ray beam, the imaging plate (100 mm radius) rotates whilst a reading head scans across the plate to measure the stimulated luminescence in a record-player-like manner. During the next period of exposure, the image taken immediately before is processed in parallel. The system has been tested using both peptide and protein crystals and has been proven to work successfully.