Effect of serum cytokines and VEGF levels on diabetic retinopathy and macular thickness
Effect of serum cytokines and VEGF levels on diabetic retinopathy and macular thickness
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发表时间：
2009-09
期刊：
影响因子：
2.2
通讯作者：
B. Ozturk;B. Bozkurt;H. Kerimoglu;M. Okka;U. Kamış;K. Gunduz
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作者：
B. Ozturk;B. Bozkurt;H. Kerimoglu;M. Okka;U. Kamış;K. Gunduz
目的探讨血清炎性细胞因子和血管内皮生长因子(VEGF)在糖尿病视网膜病变(DR)中的作用及其与光学相干断层扫描(OCT)测量黄斑厚度的关系。方法选择28例正常人(第1组)、31例非DR患者(第2组)、49例非增殖性DR患者(第3组)和46例增生性DR患者(第4组)。用OCT-3仪检测黄斑病变程度，用多重微珠免疫法检测血清血管内皮生长因子、白介素1α(IL-1α)、白介素6(IL-6)、白介素8(IL-8)、白介素10(IL-10)、巨噬细胞炎性蛋白1(MIP-1α)、单核细胞趋化蛋白-1(MCP-1)、表皮生长因子(EGF)的浓度。结果视力中位数分别为20/20(第1、2组)、20/100(第3组)、20/125(第4组)。黄斑中心亚区厚度第1组为165.50μm，第2组为202.5μm，第3组为318 pg/m，第4组为310 pg/m，第1组为98.20pg/ml，第2组为125.37 pg/ml，第3组为153.07 pg/ml，第4组为149.12 pg/ml，各组间差异有统计学意义(p0.05)。血清IL-8、IL-10、MIP-1、α和表皮生长因子水平的中位数在各组间差异较大，但组间无统计学意义(p>；0.05)。血清MCP-1浓度的中位数随糖尿病视网膜病变的进展而显著升高(p=0.02)。黄斑厚度与血清细胞因子和血管内皮生长因子水平无相关性(p>；0.05)。结论血清中高水平的血管内皮生长因子和单核细胞趋化蛋白-1可能是糖尿病视网膜病变的关键调节因子，为糖尿病患者的风险评估提供了一种潜在的工具。为了更好地了解全身和局部炎症和血管生成因子之间的相互作用，需要进一步的研究来评估不同阶段DR的玻璃体和血清水平。
Purpose To investigate the role of serum inflammatory cytokines and vascular endothelial growth factor (VEGF) in diabetic retinopathy (DR) and evaluate their relationship with macular thickness measurements obtained with optical coherence tomography (OCT). Methods The study enrolled 28 healthy subjects (Group 1), 31 patients without DR (Group 2), 49 patients with nonproliferative DR (Group 3), and 46 patients with proliferative DR (Group 4). Macular profile was assessed with Stratus OCT-3 and the serum concentrations of VEGF and interleukin-1α (IL-1α), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), macrophage inflammatory protein (MIP-1α), monocyte chemoattractant protein (MCP-1), and epidermal growth factor (EGF) were measured using multiplex bead immunoassay. Results The median value of the visual acuity was 20/20 (Groups 1 and 2), and 20/100 (Group 3), and 20/125 (Group 4). The median value of central subfield macular thickness was estimated as 165.50 μm in Group 1, 202.5 μm in Group 2, 318 μm in Group 3, and 310 μm in Group 4. The median serum VEGF level, which was 98.20 pg/ml in Group 1, demonstrated a progressive rise to 125.37 pg/ml in Group 2, to 153.07 pg/ml in Group 3, and to 149.12 pg/ml in Group 4. Statistical significance was found between all groups (p0.05). The median serum levels of IL-8, IL-10, MIP-1α, and EGF revealed a wide range within each group, however, no statistically significant relationship was found between the groups (p>0.05). The median values of the serum MCP-1 concentrations presented a statistically significant rise with the progression of DR (p=0.02). No correlation was found between macular thickness and serum cytokine and VEGF levels (p>0.05). Conclusions Increased serum levels of VEGF and MCP-1 may act as a key regulator of DR and provide a potential tool for risk assessment in diabetic patients. Further studies that evaluate both vitreous and serum levels in various stages of DR are needed to provide a better understanding of the interaction between systemic and local inflammatory and angiogenic factors.