Structure of a cluster of mouse histone genes.
Structure of a cluster of mouse histone genes.
复制标题
小鼠组蛋白基因簇的结构。
DOI：
10.1093/nar/11.19.6679
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发表时间：
1983
影响因子：
14.9
通讯作者：
Marzluff,WF
中科院分区：
文献类型：
--
作者：
Sittman,DB;Graves,RA;Marzluff,WF
已对克隆的12.9kb DNA片段中存在的四个小鼠组蛋白基因（2个H3基因、一个H2 b基因和一个H2 a基因）进行了完整的测序，包括5'和3'侧翼区。这些基因在培养的小鼠细胞中表达，并且mRNA的3'和5'末端已经通过S1核酸酶图谱确定。这些基因编码在培养的小鼠细胞中表达的组蛋白mRNA的一小部分。它们最多占每种类型总组蛋白mRNA的5-8%。两个H3基因编码H3.2和H3.1组蛋白，而H2 b基因编码在位置18处具有单个氨基酸变化（valleu）的H2b.1蛋白。克隆中仅含有H2 a基因的3'部分，并且在位置126处存在氨基酸变化（丙氨酸-脯氨酸）。5'和3'侧翼序列的比较揭示了在mRNA的3'末端的保守序列，其形成发夹环结构。基因中的密码子使用是非随机的，并且在基因的编码区中对CG双联体没有歧视。
The four mouse histone genes(2 H3 genes, an H2b gene and an H2a gene) present in a cloned 12.9 kilobase fragment of DNA have been completely sequenced including both 5' and 3' flanking regions. These genes are expressed in cultured mouse cells and the 3' and 5' ends of the mRNA have been determined by S1 nuclease mapping. These genes code for a minor fraction of the histone mRNAs expressed in cultured mouse cells. They comprise at most 5–8% of the total histone mRNA of each type. The two H3 genes code for H3.2 and H3.1 histone proteins, while the H2b gene codes for an H2b.1 protein with a single amino acid change (valleu) at position 18. Only the 3' portion of the H2a gene is contained in the clone and there is an amino acid change (alanine - proline) at position 126. Comparison of the 5' and 3' flanking sequences reveals a conserved sequence at the 3' end of the mRNA which forms a hairpin loop structure. The codon usage in the genes is non-random and there has been no discrimination against CG doublets in the coding region of the genes.