Floral organs act as environmental filters and interact with pollinators to structure the yellow monkeyflower (Mimulus guttatus) floral microbiome
Floral organs act as environmental filters and interact with pollinators to structure the yellow monkeyflower (Mimulus guttatus) floral microbiome
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DOI：
10.1111/mec.15280
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发表时间：
2019-11-12
影响因子：
4.9
通讯作者：
Ashman, Tia-Lynn
中科院分区：
文献类型：
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作者：
Gomez, Maria Rebolleda;Ashman, Tia-Lynn
微生物群落的组装是中性和选择性过程的结果。然而，这些过程的相对重要性仍有争议。特别是花的微生物群落，由于其对植物适应性和植物-传粉者相互作用的潜在影响而获得了最近的关注。然而，选择和分散在这些社区的集会的作用仍然知之甚少。在这里，我们评估了传粉媒介介导的传播的作用，中性和选择性的过程中的黄色monkeyflower（Mimulus guttatus）的花微生物组的组装的贡献。我们采样花器官的存在和不存在的传粉者在五个不同的蛇形渗漏在CA的花，并获得16 S扩增子数据的附生细菌群落。与强大的微环境选择花内，我们观察到显着差异，在整个花器官的社区组成，只有一个小的影响，地理距离。传粉者暴露影响环境选择的贡献，并依赖于与花游客的互动率和亲密度。这项研究提供了证据的分散和花内异质性的重要性，在塑造附生细菌群落的花，并强调传粉者的行为，环境选择和额外的非生物因素之间的复杂的相互作用，在塑造附生细菌群落的花。
Assembly of microbial communities is the result of neutral and selective processes. However, the relative importance of these processes is still debated. Microbial communities of flowers, in particular, have gained recent attention because of their potential impact to plant fitness and plant-pollinator interactions. However, the role of selection and dispersal in the assembly of these communities remains poorly understood. Here, we evaluated the role of pollinator-mediated dispersal on the contribution of neutral and selective processes in the assembly of floral microbiomes of the yellow monkeyflower (Mimulus guttatus). We sampled floral organs from flowers in the presence and absence of pollinators within five different serpentine seeps in CA and obtained 16S amplicon data on the epiphytic bacterial communities. Consistent with strong microenvironment selection within flowers we observed significant differences in community composition across floral organs and only a small effect of geographic distance. Pollinator exposure affected the contribution of environmental selection and depended on the rate and intimacy of interactions with flower visitors. This study provides evidence of the importance of dispersal and within-flower heterogeneity in shaping epiphytic bacterial communities of flowers, and highlights the complex interplay between pollinator behaviour, environmental selection and additional abiotic factors in shaping the epiphytic bacterial communities of flowers.