Genome-wide array-based comparative genomic hybridization of diffuse large B-cell lymphoma: Comparison between CD5-positive and CD5-negative cases
Genome-wide array-based comparative genomic hybridization of diffuse large B-cell lymphoma: Comparison between CD5-positive and CD5-negative cases
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DOI：
10.1158/0008-5472.can-03-4056
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发表时间：
2004-09-01
期刊：
影响因子：
11.2
通讯作者：
Seto, M
中科院分区：
文献类型：
--
作者：
Tagawa, H;Tsuzuki, S;Seto, M
弥漫性大B细胞淋巴瘤(DLBCL)是最常见的非霍奇金淋巴瘤，临床表现出侵袭性和异质性。为了确定DLBCL的遗传学特征，我们建立了自己的基于阵列的比较基因组杂交，共分析了70例DLBCL[26例CD阳性(CD5(+))DLBCL和44例CD5阴性(CD5(-))DLBCL]。CD5(+)和CD5(-)组中均有20%的病例基因组异常区域为1q21-q31、1q32、3p25-q29、5p13、6p21-p25、7p22-q31、8q24、11q23-q24、12q13-q21、16p13、18和X，1p36、3p14、6q14-q25、6q27、9p21和17p11-p13缺失。由于CD5的表达标志着预后不良的亚组，我们随后分析了CD5(+)DLBCL和CD5-DLBCL的基因组得失。虽然两组的得失具有相似的基因组模式，但10p14-p15和19q13的获得以及1q43-q44和8p23的丢失是CD5(+)DLBCL的特征。通过关注13q21-q34的获得和1p34-p36的丢失，我们还能够在CD5(+)DLBCL病例中识别不同的预后亚群。这些结果表明，基于阵列的比较基因组杂交分析为DLBCL的基因组异常提供了一个平台，既有临床上不同亚群的常见的，也有特定的。
Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma and exhibits aggressive and heterogeneous clinical behavior. To genetically characterize DLBCL, we established our own array-based comparative genomic hybridization and analyzed a total of 70 cases [26 CD-positive (CD5(+)) DLBCL and 44 CD5-negative (CD5(-)) DLBCL cases]. Regions of genomic aberrations observed in >20% of cases of both the CD5(+) and CD5(-) groups were gains of 1q21-q31, 1q32, 3p25-q29, 5p13, 6p21-p25, 7p22-q31, 8q24, 11q23-q24, 12q13-q21, 16p13, 18, and X and losses of 1p36, 3p14, 6q14-q25, 6q27, 9p21, and 17p11-p13. Because CD5 expression marks a subgroup with poor prognosis, we subsequently analyzed genomic gains and losses of CD5(+) DLBCL compared with those of CD5-. Although both groups showed similar genomic patterns of gains and losses, gains of 10p14-p15 and 19q13 and losses of 1q43-q44 and 8p23 were found to be characteristic of CD5(+) DLBCL. By focusing on the gain of 13q21-q34 and loss of 1p34-p36, we were also able to identify prognostically distinct subgroups among CD5(+) DLBCL cases. These results suggest that array-based comparative genomic hybridization analysis provides a platform of genomic aberrations of DLBCL both common and specific to clinically distinct subgroups.