Deferoxamine iron chelation increases delta-aminolevulinic acid induced protoporphyrin IX in xenograft glioma model.
Deferoxamine iron chelation increases delta-aminolevulinic acid induced protoporphyrin IX in xenograft glioma model.
复制标题
去铁胺铁螯合可增加异种移植神经胶质瘤模型中 δ-氨基乙酰丙酸诱导的原卟啉 IX。
DOI：
10.1111/j.1751-1097.2009.00664.x
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发表时间：
2010
影响因子：
3.3
通讯作者：
Pogue,BrianW
中科院分区：
文献类型：
--
作者：
Valdés,PabloA;Samkoe,Kimberley;O'Hara,JuliaA;Roberts,DavidW;Paulsen,KeithD;Pogue,BrianW
δ-氨基乙酰丙酸（δ-ALA）的外源性给药导致脑肿瘤中原卟啉IX（PpIX）的选择性积累，并在脑肿瘤的荧光引导切除术（FGR）中显示出增加切除程度的有希望的结果。然而，这种方法仍然受到异质染色的影响，因此由于PpIX产生的这种变化，一些肿瘤边缘可能无法检测到。本研究的目的是验证铁螯合治疗可以增加恶性胶质瘤肿瘤中荧光水平的假设。在δ-ALA给药前，对植入异种移植物U251-GFP胶质瘤肿瘤细胞的小鼠给予200 mg kg-1剂量的去铁胺（DFO），每天一次，持续3天。 在没有DFO的动物组中，在肿瘤区域中观察到的PpIX荧光是背景的1.9倍，在DFO预处理组中，平均是背景的2.9倍。相对于对照组，观察到DFO组中PpIX荧光对比度增加50%（t检验P值= 0.0020）。 这些结果表明，铁螯合疗法可以显著增加恶性胶质瘤中δ-ALA-诱导的PpIX荧光，指出铁螯合疗法对于脑肿瘤更有效的FGR的潜在作用。
Exogenous administration of δ‐aminolevulinic acid (δ‐ALA) leads to selective accumulation of protoporphyrin IX (PpIX) in brain tumors, and has shown promising results in increasing extent of resection in fluorescence‐guided resection (FGR) of brain tumors. However, this approach still suffers from heterogeneous staining and so some tumor margins may go undetected because of this variation in PpIX production. The aim of this study was to test the hypothesis that iron chelation therapy could increase the level of fluorescence in malignant glioma tumors. Mice implanted with xenograft U251‐GFP glioma tumor cells were given a 200 mg kg−1dose of deferoxamine (DFO), once a day for 3 days prior to δ‐ALA administration. The PpIX fluorescence observed in the tumor regions was 1.9 times the background in animal group without DFO, and 2.9 times the background on average, in the DFO pre‐treated group. A 50% increase in PpIX fluorescence contrast in the DFO group was observed relative to the control group (t‐testP‐value = 0.0020). These results indicate that iron chelation therapy could significantly increase δ‐ALA‐induced PpIX fluorescence in malignant gliomas, pointing to a potential role of iron chelation therapy for more effective FGR of brain tumors.