DETECTION OF ANTIGENS OF LARVAL TAENIA-SOLIUM IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH THE USE OF HPLC AND ELISA
DETECTION OF ANTIGENS OF LARVAL TAENIA-SOLIUM IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH THE USE OF HPLC AND ELISA
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DOI：
10.2307/3282629
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发表时间：
1990-02-01
影响因子：
1.3
通讯作者：
KUHN, RE
中科院分区：
文献类型：
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作者：
CHOROMANSKI, L;ESTRADA, JJ;KUHN, RE
使用高效液相色谱凝胶过滤柱分离从疑似患有神经囊虫病的患者（79个样品）以及从对照患者（无神经系统症状）获得的脑脊液（CSF）。在色谱分离过程中，根据特征峰分别收集洗脱级分。通过对齐100多个色谱图和6个特征峰，在一致的位置洗脱，鉴定了CSF组分的洗脱特征。在酶联免疫吸附试验（ELISA）中检测每个峰的样品中是否存在幼虫抗原。在79例疑似病例中，发现44例脑脊液中含有幼虫抗原，这些抗原在2号峰中检出，其平均分子量约为110，000。此外，在某些情况下，在1号峰中发现了幼虫抗原;然而，在44份CSF样品中，我们仅在23份CSF样品中检测到了幼虫抗原，其中幼虫抗原存在于2号峰中。这23份CSF样本中的9份（来自79例疑似脑囊虫病的患者）后来通过组织病理学证实。来自对照患者的5个CSF样品的ELISA读数值（0.054 ± 0.055）。0.064）被认为是阴性的。因此，在来自疑似患有脑囊虫病的患者的79个CSF样品中的44个中，ELISA值是高度阳性的（0.551 ± 0.001）。0.293）。其余35份CSF样品的ELISA读数为0.092 ±。 0.062，这与对照患者的CSF获得的值没有显著差异。本研究的结果表明，斑点和/或标准ELISA可以通过检测患者CSF中猪带绦虫幼虫抗原的存在来辅助诊断脑囊虫病。
The cerebrospinal fluid (CSF) obtained from patients suspected of having neurocysticercosis (79 samples), as well as from control patients (without neurological symptoms), was separated using a high performance liquid chromatography gel filtration column. During the chromatographic separation, the eluted fractions were collected separately according to distinctive peaks. The elution characteristics of CSF components were identified by aligning more than 100 chromatograms and 6 distinctive peaks, eluting in consistent positions. Samples of each peak were tested in an enzyme-linked immunosorbent assay (ELISA) for the presence of larval antigens. Forty-four of the suspected 79 cases were found to have larval antigens in their CSF and these antigens were detected in peak no. 2, the mean of which is approximately 110,000 molecular weight. Also, in some cases, larval antigens were found in peak no. 1; however, we were able to detect them in only 23 CSF samples out of 44 CSF samples in which larval antigens were present in peak no. 2. Nine of these 23 CSF samples (derived from 79 patients in which neurocysticercosis was suspected) were later confirmed by histopathology. Values of ELISA readings of 5 CSF samples from control patients (0.054 .+-. 0.064) were considered negative. Thus, in 44 of 79 CSF samples from patients suspected of having neurocysticercosis, the ELISA values were highly positive (0.551 .+-. 0.293). The remaining 35 CSF samples gave ELISA readings of 0.092 .+-. 0.062, which were not significantly different from values obtained with CSF of control patients. The results of this study demonstrate that dot and/or standard ELISA can aid in the diagnosis of neurocysticercosis by detecting the presence of antigens of Taenia solium larvae in the CSF of patients.