The involvement of neurokinin receptor subtypes in somatosensory processing in the superficial dorsal horn of the cat
The involvement of neurokinin receptor subtypes in somatosensory processing in the superficial dorsal horn of the cat
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神经激肽受体亚型参与猫浅背角体感处理
DOI：
10.1016/0006-8993(90)90075-m
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
C. Bladon
中科院分区：
文献类型：
--
作者：
S. Fleetwood;R. Mitchell;P. J. Hope;N. El;V. Molony;C. Bladon
除了P物质（SP）外，神经激肽A （NKA）和神经激肽B （NKB）最近在脊髓浅表背角中被发现；NKA主要起源于细小的初级传入。我们已经研究了这些速激素和一系列类似物对单个鉴定的背角神经元的体感觉反应的影响，当离子电泳应用于明胶质区域时。热伤害感觉的行为反射测试同时进行。讨论了NK-1、NK-2和NK-3受体的作用。nk -1选择性激动剂可减弱经鉴定的多感受性脊髓脊髓束（SCT）神经元的非伤害性反应。在内源性速激肽中，SP和NKB（一种弱NK-1激动剂）均表现出这种作用。在我们的实验中没有发现NK-3受体的作用。nk -2选择性激动剂（包括NKA）引起独特的选择性促进热伤害反应。NKA还减少了甩尾和热板测试中的反射反应潜伏期。因此，NKA作为主要传入递质可能参与介导或促进明胶物质中热伤害性输入的表达。NKA和SP可被认为在浅表背角中协同作用，有效地起着促进伤害的调节作用。受体选择性拮抗剂的证据支持激动剂获得的特定NK受体在体感觉加工中的作用。NK-2，而不是NK-1或NK-3拮抗剂能减弱内源性热伤害性反应，这支持了NK-2激动剂（如NKA）可能通常参与浅背角热伤害性表达的假设。行为实验显示，对假定的NK-2选择性拮抗剂的反应潜伏期增加，进一步支持NK-2受体参与热伤害感受。
As well as substance P (SP), neurokinin A (NKA) and neurokinin B (NKB) have recently been found in the superficial dorsal horn of the spinal cord; NKA originating mainly in fine primary afferents. We have investigated the effects of these tachykinins and a range of analogues on somatosensory responses of single identified dorsal horn neurons, when applied ionophoretically to the region of the substantia gelatinosa. Behavioural reflex tests of thermal nociception were carried out in parallel. The role of NK-1, NK-2 and NK-3 receptors was addressed. NK-1-selective agonists attenuated the non-nociceptive responses of identified multireceptive spinocervical tract (SCT) neurons. Of the endogenous tachykinins, both SP and NKB (a weak NK-1 agonist) showed this effect. No role for NK-3 receptors was identified in our experiments. NK-2-selective agonists (including NKA) caused a unique and selective facilitation of thermal nociceptive responses. NKA also reduced reflex response latency in tail-flick and hot plate tests. NKA as a primary afferent transmitter may thus be involved in mediating or facilitating the expression of thermal nociceptive inputs in the substantia gelatinosa. NKA and SP could be considered as acting in concert in the superficial dorsal horn in an effectively pro-nociceptive modulatory role. Evidence from receptor-selective antagonists supports that obtained with agonists for the roles of particular NK receptors in somatosensory processing. NK-2, but not NK-1 or NK-3 antagonists attenuated endogenous thermal nociceptive responses, supporting the hypothesis that an NK-2 agonist (such as NKA) may normally participate in expression of thermal nociception in the superficial dorsal horn. Behavioural experiments showing increased response latencies with a putative NK-2 selective antagonist further supported the involvement of NK-2 receptors in thermal nociception.