Regulation of cell volume in separated renal tubules incubated in hypotonic medium.
Regulation of cell volume in separated renal tubules incubated in hypotonic medium.
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在低渗培养基中孵育的分离肾小管中细胞体积的调节。
DOI：
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发表时间：
1979
影响因子：
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通讯作者：
J. Gardin
中科院分区：
文献类型：
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作者：
M. Paillard;F. Leviel;J. Gardin
本文研究了胶原酶去除基底膜的分离肾小管(SRT)细胞体积的调节。当SRT浸泡在低渗介质中时，细胞水分含量的增加是在没有调控的情况下预期的一半。调节是立即的，最初没有肿胀，伴随着盐分的丧失，细胞K没有变化。这种调节被10(-3)M哇巴因消除。我们得出的结论是：1)在低渗介质中发生的细胞体积调节是由于立即失去了氯化钠。2)氯化钠的损失可能是由于跨膜氯化钠电化学梯度的下降，阻止了被动的氯化钠净进入细胞。高的膜钠通透性，可能位于肾小管细胞的管腔侧，可能解释了为什么调节是瞬时的。3)哇巴因取消体积调节表明，没有必要假设哇巴因不敏感的泵调节细胞体积。
The regulation of cell volume was studied in separated renal tubules (SRT) whose basement membrane had been removed by collagenase. Regulation occurred when SRT were immersed in a hypotonic medium, the increase in cellular water content being half that expected in the absence of regulation. Regulation was immediate, with no initial swelling, and was accompanied by a loss of NaCl, with no change in cellular K. This regulation was eliminated by 10(-3) M ouabain. We conclude that: 1) Cell volume regulation which occurs in a hypotonic medium is due to an immediate loss of NaCl. 2) Loss of NaCl might be due to blocking of the net passive NaCl entry into the cells resulting from the drop in the transmembrane NaCl electrochemical gradient. The high membrane sodium permeability, probably located on the luminal side of the tubular cells, might explain why regulation was instantaneous. 3) Elimination of volume regulation by ouabain suggests there is no need to assume that a ouabain-insensitive pump regulates cell volume.