Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and tack of protective gene expression in the cornified envelope
Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and tack of protective gene expression in the cornified envelope
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DOI：
10.1111/j.1365-2133.2005.06352.x
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发表时间：
2005-01-01
影响因子：
10.3
通讯作者：
Kimura, T
中科院分区：
文献类型：
--
作者：
Sugiura, H;Ebise, H;Kimura, T
背景特应性皮炎（AD）特异性基因尚未阐明。目的研究活动性特应性皮肤病变的特异性基因表达。方法应用Affyphin寡核苷酸芯片分析了17例AD患者和4例正常对照的23 000个基因。结果在10个基因中，S100 A8和S100 A7在AD患者和正常对照中表达差异最大，而S100 A8和S100 A7在AD患者和正常对照中表达差异最大。结论S100 A8和S100 A7基因在AD患者和正常对照中表达差异最大（上调），以及兜甲蛋白和聚丝蛋白（下调），表皮分化基因位于1q21，一个位点以前报道有遗传连锁与AD。结论我们的研究结果，显示下调的皮质包膜基因和上调的替代角化途径，首次提出异常表皮分化和防御缺陷是AD的关键异常。
Background Atopic dermatitis (AD)-specific genes have not yet been clarified. Objectives To identify gene expression specific to active atopic skin lesions.Methods We analysed 23 000 genes in skin biopsy samples from 17 patients with AD and four normal controls using Affymetrix oligonucleotide arrays.Results Four of the 10 genes with the greatest differences in expression between patients and controls, S100A8 and S100A7 (upregulated), and loricrin and filaggrin (downregulated), were epidermal differentiation genes located on 1q21, a locus previously reported to have a genetic linkage with AD.Conclusions Our results, showing downregulation of the cornified envelope genes and upregulation of the alternative keratinization pathway, are the first to suggest abnormal epidermal differentiation and defective defences as key abnormalities in AD.