Domestic allergens in public places .2. Dog (Can f 1) and cockroach (Bla g 2) allergens in dust and mite, cat, dog and cockroach allergens in the air in public buildings
Domestic allergens in public places .2. Dog (Can f 1) and cockroach (Bla g 2) allergens in dust and mite, cat, dog and cockroach allergens in the air in public buildings
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DOI：
10.1111/j.1365-2222.1996.tb00521.x
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发表时间：
1996-11-01
影响因子：
6.1
通讯作者：
Woodcock, A
中科院分区：
文献类型：
--
作者：
Custovic, A;Green, R;Woodcock, A
背景致敏和暴露于室内过敏原是哮喘的主要危险因素。目的调查公共建筑和公共交通工具中地毯和软垫座椅灰尘中Can f 1和Bla g 2的水平，以及空气中Der p 1、Fel d 1、在学校和办公室的Can f 1和Bla g 2。方法在5所学校，6家酒店，四家电影院，六家酒吧，三辆公共汽车和两列火车。还从同一地区20个有狗和20个没有狗的家庭的卧室地毯、客厅地毯、床垫和沙发上收集了灰尘。在办公室（n = 16）和教室（n = 9）进行个人空气采样（2 L/min）8小时。此外，空气中的样本在学校收集使用高容量泵（60升/分钟）1小时后，立即在三个教室的孩子离开学校。结果所有公共场所粉尘样品均检出Can f1，范围为0.2 ~ 52.5 μ g/g。在软垫座椅中发现的含量（几何平均值- GM 9.4 μ g/g）明显高于地毯（GM 1.5 μ g/g; P 10 μ g/g），在公共场所的软垫座椅中发现了40%。在公共场所的软垫座椅中，Can f1显著高于没有狗的家庭中的沙发（GM 1.8 μ g/g; P < 0.001）。在所有学校都发现了可检测的Bla g 2水平（GM 2.4 U/g，范围0.8-4.4 U/g）。在65%的抽样教室中测得Bla g 2浓度大于2U/g（代表致敏风险的临时阈值水平）。Der p 1和Bla g 2在所有空气样品中均低于检测限。然而，空气中的Fel D 1和可以f 1在学校和办公室中检测到，虽然在低concentration.Conclusions软垫座椅从公共场所构成的水库狗过敏原的积累，并在公共建筑物内或公共交通工具上的暴露源可以f 1。在学校接触蟑螂过敏原可能对蟑螂致敏的哮喘儿童很重要。
Background Sensitization and exposure to indoor allergens are the major risk factors for asthma. It is possible that significant exposure to domestic allergens occurs outside the home.Objectives To investigate the levels of Can f 1 and Bla g 2 in the dust from carpeted floors and upholstered seats in public buildings and public transport and the airborne concentrations of Der p 1, Fel d 1, Can f 1 and Bla g 2 in schools and offices.Methods Can f 1 and Bla g 2 were measured in the dust collected by vacuuming a 1 m(2) area of carpet, as well as upholstered seats in five schools, six hotels, four cinemas, six pubs, three buses and two trains. Dust was also collected from the bedroom carpet, living room carpet, mattress and sofa in 20 homes with and 20 homes without a dog in the same area. Personal airborne sampling (2 L/min) was conducted for 8 h in offices (n = 16) and classrooms (n = 9). In addition, airborne samples in schools were collected using a high volume pump (60 L/min) for 1 h in three classrooms immediately after the children vacated the school. Can f 1, Bla g 2, Der p 1 and Fel d 1 were assayed using a two-site monoclonal antibody-based ELISA.Results Can f 1 was detected in all dust samples from public places, ranging from 0.2 to 52.5 mu g/g. Significantly higher levels were found in upholstered seats (geometric mean - GM 9.4 mu g/g) than in carpets (GM 1.5 mu g/g; P 10 mu g/g were found in 40% of upholstered seats in public places. Can f 1 was significantly higher in upholstered seats in public places than in sofas in homes without a dog (GM 1.8 mu g/g; P < 0.001). Detectable levels of Bla g 2 were found in all of the schools (GM 2.4 U/g, range 0.8-4.4 U/g). Bla g 2 concentration greater than 2U/g (provisional threshold level representing risk of sensitization) was measured in 65% of the classrooms sampled. Der p 1 and Bla g 2 were below the detection limit in all airborne samples. However, airborne Fel d 1 and Can f 1 were detected in schools and offices, albeit in low concentrations.Conclusions Upholstered seats from public places constitute a reservoir for the accumulation of dog allergen, and a source of exposure to Can f 1 inside public buildings or on public transport. Exposure to cockroach allergens in schools may be important for cockroach sensitized asthmatic children.