Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets
Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets
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DOI：
10.1016/j.vetmic.2007.08.004
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发表时间：
2008-02-05
影响因子：
3.3
通讯作者：
Torres, Carmen
中科院分区：
文献类型：
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作者：
Costa, Daniela;Poeta, Patricia;Torres, Carmen
将从葡萄牙北部采集的健康狗(n=39)和猫(n=36)的粪便样本接种在Levine琼脂平板上，每个样本回收两个大肠杆菌分离物(狗78个，猫66个)。对144株大肠埃希菌进行了16种抗菌药物的药敏试验。近20%的菌株对四环素耐药，12%和15%的菌株对氨苄西林和链霉素耐药。对其他抗菌药物的耐药率均在4%以下，未检出头孢他啶、亚胺培南、头孢西丁和阿米卡星耐药株。两个分离株(来自一只狗)对头孢噻肟耐药，同时含有CTX-M-1和OXA-30β-内酰胺酶。17株氨苄西林耐药株中12株检测到blA基因，3株庆大霉素耐药株检测到aac(3)-II基因，22株链霉素耐药株中7株检测到aadA基因，28株四环素耐药株全部检测到tet(A)和/或tet(B)基因。在4株新诺明耐药株和2株CTX-M-1和OXA-30β-内酰胺酶分离株中检测到I类整合酶基因，发现dfrA1+aadA1(2株)、dfrA12+orff+aadA2(2株)和bla(OXA30)+aadA1(2株)。在4株奈利地克酸耐药和环丙沙星敏感株中，GyrA蛋白(Ser83Leu或Asp87Tyr)有1个氨基酸发生改变，在1株奈利地酸和环丙沙星耐药株中有2个GyrA(Ser83Leu+Asp87Asn)和1个ParC(Ser80I1e)氨基酸发生改变。健康宠物的粪便大肠杆菌分离株可能是抗菌素耐药性基因的储存库。(C)2007 Elsevier B.V.保留所有权利。
Faecal samples of healthy dogs (n = 39) and cats (n = 36) obtained in Northern Portugal were seeded on Levine agar plates, and two Escherichia coli isolates per sample were recovered (78 of dogs and 66 of cats). The susceptibility to 16 antimicrobial agents was tested in this series of 144 E. coli isolates. Almost 20% of them showed tetracycline resistance and 12 and 15% presented ampicillin or streptomycin resistance, respectively. The percentage of resistance to the other antimicrobial agents was in all cases below 4%, and no resistant isolates were detected for ceftazidime, imipenem, cefoxitin or amikacin. Two isolates (from one dog) showed cefotaxime-resistance and harboured both the CTX-M-1 and OXA-30 beta-lactamases. A bla(TEM) gene was detected in 12 of 17 ampicillin-resistant isolates, the aac(3)-II gene in the three gentamicin-resistant isolates, aadA in 7 of 22 streptomycin-resistant isolates, and tet(A) and/or tet(B) gene in all 28 tetracycline-resistant isolates. The gene encoding class I integrase was detected in six E. coli isolates, including the four trimethoprim-sulfamethoxazole-resistant isolates and those two harbouring CTX-M-1 and OXA-30 beta-lactamases; different gene cassette arrangements were identified: dfrA1 + aadA1 (two isolates), dfrA12 + orfF + aadA2 (two isolates) and bla(OXA30) + aadA1 (two isolates). One amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in four nalidixic acid-resistant and ciprofloxacin-susceptible isolates and two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80I1e) were identified in one nalidixic acid- and ciprofloxacin-resistant isolate. Faecal E. coli isolates of healthy pets could be a reservoir of antimicrobial resistance genes. (c) 2007 Elsevier B.V. All rights reserved.