Direct microscopy of periodontal pathogens.
Direct microscopy of periodontal pathogens.
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牙周病原体的直接显微镜检查。
DOI：
10.1111/j.1399-302x.1986.tb00314.x
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发表时间：
1986
影响因子：
--
通讯作者：
Listgarten,MA
中科院分区：
文献类型：
--
作者：
Listgarten,MA
牙周微生物群的显微镜研究是最早证明存在与牙周疾病和牙周健康的各种状态相关的独特微生物群的研究之一。这些发现导致了进一步的研究，表明传统的机械清创术能够使牙周微生物群的组成从疾病转变为健康相关。对选定牙齿表面某些细菌形态型分布的调查证实，健康和患病部位之间的微生物群组成存在显着差异;然而，同一受试者中类似探测深度的牙周袋的微生物群比其他受试者中类似牙周袋的微生物群更相似。这些发现支持使用来自具有相似临床特征的选定表面的合并微生物样本。以这种方式汇集的微生物样本的基础上，有可能证明在以前治疗牙周炎的患者中，那些具有高比例的活动杆和螺旋体的患者比这些细菌形态型比例低的受试者更可能发展为随后的牙周炎复发。在定期接受牙周炎治疗的患者中，这种显微镜检测的预测可靠性明显恶化。这一观察结果表明，如果检测对象很容易受到环境因素的影响，而环境因素不会以平行方式影响受试者对疾病的易感性，则在使用潜在的诊断检测时应谨慎。
Microscopic studies of the periodontal microbiota were among the first to demonstrate the existence of distinctive microbial populations in association with various states of periodontal disease and periodontal health. These findings led to further studies which indicated that conventional mechanical debridement is able to create shifts in the composition of the periodontal microbiota from a disease‐ to a health‐associated one. Investigations of the distribution of certain bacterial morphotypes on selected tooth surfaces confirmed that significant differences exist in the composition of the microbiota between healthy and diseased sites; however, periodontal pockets of similar probing depths in the same subject had microbiotas that were more similar lo one another than to those of comparable pockets in other subjects. These findings lend support to the use of pooled microbial samples from selected surfaces with similar clinical characteristics. On the basis of microbial samples pooled in this manner, it was possible to demonstrate in patients previously treated for periodontitis, that those with elevated proportions of motile rods and spirochetes were more likely to develop subsequent recurrences of periodontitis than subjects with low proportions of these bacterial morphotypes. The predictive reliability of this microscopic assay markedly worsened in patients receiving regularly scheduled periodontal prophylaxes. This observation suggests that caution should be exercised in the utilization of potential diagnostic tests, if the object of the assay can be readily affected by environmental factors that do not affect the subject's susceptibility lo disease in a parallel manner.