CHARACTERIZATION OF THE HLYB GENE AND ITS ROLE IN THE PRODUCTION OF THE EL-TOR HEMOLYSIN OF VIBRIO-CHOLERAE O1
CHARACTERIZATION OF THE HLYB GENE AND ITS ROLE IN THE PRODUCTION OF THE EL-TOR HEMOLYSIN OF VIBRIO-CHOLERAE O1
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DOI：
10.1111/j.1365-2958.1990.tb00608.x
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发表时间：
1990-03-01
影响因子：
3.6
通讯作者：
MANNING, PA
中科院分区：
文献类型：
--
作者：
ALM, RA;MANNING, PA
El - Tor霍乱弧菌菌株能够产生溶血素，并将其主动分泌到生长培养基中。这需要翻译在细胞质膜表面产生蛋白质，并在细胞膜、质周间隙和外膜之间进行转运。这种现象发生的机制尚不清楚。除了溶血素（hlyA）的结构基因外，我们还克隆了第二个邻近基因hlyB。通过定点诱变，构建了特异的hlyB突变体。这些突变体在生长的早期到中期指数期分泌HlyA有缺陷，溶血素被困在细胞内，只在固定期释放。核苷酸序列分析和细胞分离表明HlyB是一个60.3 kD的推测外膜相关蛋白。
El Tor strains of Vibrio cholerae are capable of producing a haemolysin which they actively secrete into the growth medium. This requires translation to produce the protein at the surface of the cytoplasmic membrane and translocation across this membrane, the periplasmic space and the outer membrane. The mechanism by which this occurs is poorly understood. In addition to the structural gene for the haemolysin (hlyA), we have cloned a second adjacent gene, hlyB. By site-directed mutagenesis, specific hlyB mutants have been constructed. These mutants are defective in the secretion of HlyA in the early to mid-exponential phase of growth and the haemolysin becomes trapped within the cell and is only released in stationary phase. Nucleotide sequence analysis and cell fractionations reveal HlyB to be a 60.3 kD putative outer membrane-associated protein.