Correlation analysis of gap junction lattice images.
Correlation analysis of gap junction lattice images.
复制标题
间隙连接晶格图像的相关分析。
DOI：
10.1016/s0006-3495(90)82462-0
复制
发表时间：
1990
影响因子：
3.4
通讯作者：
Goodenough,DA
中科院分区：
文献类型：
--
作者：
Sosinsky,GE;Baker,TS;Caspar,DL;Goodenough,DA
从低辐射电子显微照片计算孤立的负染色间隙连接域的连接子图像的傅立叶平均值表现出染色分布和连接子方向的差异。为了分析这些多态性结构，相关平均方法被应用到负染色和冷冻水合标本的图像。对于阴性染色的标本，获得了轴向通道中具有不同程度染色累积的两个连接子子集的单独平均值。在冷冻水合样本的单个交界区域内，具有相反倾斜方向的两个连接子群体是可以区分的。使用左偏和右偏的参考文献计算的相关图显示，所选的连接子倾向于局部聚类。使用相关性方法来分析一个典型的连接子晶格中的堆积无序，我们估计最近邻对分离的均方根变化约为晶格常数的11%。位移的连接子相对于彼此的增加对分离的晶格中，而像一种液体，虽然长程取向顺序是保守的晶体。这些结果支持的假设，六方有序的连接子的结果从短程排斥力。
Fourier averages of connexon images computed from low-irradiation electron micrographs of isolated negatively stained gap junction domains exhibited differences in stain distribution and connexon orientation. To analyze these polymorphic structures, correlation averaging methods were applied to images from negatively stained and frozen-hydrated specimens. For the negatively stained specimens, separate averages over two subsets of connexons with differing degrees of stain accumulation in the axial channel were obtained. Two populations of connexons with opposite skew orientations were distinguishable within a single junctional domain of a frozen-hydrated specimen. Correlation maps calculated using the left- and right-skewed references showed that the selected connexons tend to locally cluster. Using correlation methods to analyze packing disorder in a typical connexon lattice, we estimated the root-mean-square variation in the nearest neighbor pair separation to be approximately 11% of the lattice constant. Displacements of the connexons relative to each other increased with increasing pair separation in the lattice, rather like a liquid, although long-range orientation order was conserved as in a crystal. These results support the hypothesis that the hexagonal ordering of the connexons results from short-range repulsive forces.