Inhibition of Colony Formation of Drug‐resistant Human Tumor Cell Lines by Combinations of Interleukin‐2‐activated Killer Cells and Antitumor Drugs
Inhibition of Colony Formation of Drug‐resistant Human Tumor Cell Lines by Combinations of Interleukin‐2‐activated Killer Cells and Antitumor Drugs
复制标题
Interleukin-2激活的杀伤细胞与抗肿瘤药物联合抑制耐药人肿瘤细胞系集落形成
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
N. Saijo
中科院分区：
文献类型：
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作者：
A. Ohtsu;Y. Sasaki;T. Tamura;Y. Fujiwara;Y. Ohe;K. Minato;K. Nakagawa;M. Bungo;N. Saijo
通过集落测定法，体外评价了白细胞介素2激活的杀伤（LAK）细胞在有或无抗癌药物的情况下对获得性耐药细胞系的细胞毒性。使用人非小细胞肺癌细胞系PC-9和PC-14、人白血病细胞系K-562及其对顺铂（CDDP）耐药的亚系PC-9/CDDP和PC-14/CDDP以及对阿霉素（ADM）耐药的亚系K-562/ADM作为靶细胞。与亲本细胞系PC-9相比，PC-9/CDDP对外周血淋巴细胞（PBL）和LAK细胞杀伤的敏感性显著增加。PBL和LAK细胞对PC-14/CDDP和K-562/ADM的细胞毒作用与其亲本细胞相似。此外，LAK与CDDP联合应用对PC-14和PC-14/CDDP具有协同作用。
The cytotoxicity of interleukin‐2‐activated killer (LAK) cells with or without anticancer drugs against cell lines with acquired drug resistance was evaluated in vitro by colony assay. Human non‐small cell lung cancer cell lines, PC‐9 and PC‐14, human leukemia cell line, K‐562, and their sublines resistant to cisplatin (CDDP), PC‐9/CDDP and PC‐14/CDDP, and to adriamycin (ADM), K‐562/ADM, were used as target cells. PC‐9/CDDP demonstrated a marked increase in susceptibility to killing by both peripheral blood lymphocytes (PBL) and LAK cells, as compared to the parental cell line, PC‐9. The cytotoxicity of PBL and LAK cells against PC‐14/CDDP and K‐562/ADM was similar to that against their parental cell lines. Moreover, the combination of LAK and CDDP had a synergistic effect on PC‐14 and PC‐14/CDDP.
多效耐药人红白血病 (K562-R) 细胞系对自然杀伤细胞介导的细胞溶解的抵抗。
影响因子：
11.2
作者：
Yanovich,S;Hall,RE;Weinert,C
通讯作者：
Weinert,C