Propagation of field highly pathogenic porcine reproductive and respiratory syndrome virus in MARC-145 cells is promoted by cell apoptosis
Propagation of field highly pathogenic porcine reproductive and respiratory syndrome virus in MARC-145 cells is promoted by cell apoptosis
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DOI：
10.1016/j.virusres.2015.12.023
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发表时间：
2016-02-02
期刊：
影响因子：
5
通讯作者：
Zeng, Fanya
中科院分区：
文献类型：
--
作者：
Ge, Mengyun;Zhang, Yi;Zeng, Fanya
猪繁殖与呼吸综合征病毒(PRRSV)感染在体内和体外均可诱导细胞凋亡。然而，宿主细胞凋亡和PRRSV复制之间的关系尚不清楚。本文报道了细胞凋亡对PRRSV在MARC-145细胞中增殖的促进作用。对野生型高致病性PRRSV(HP-PRRSV)在MARC-145细胞中繁殖的观察表明，过度生长的MARC-145细胞感染后病毒产量明显增加，并在接种病毒前触发了细胞的凋亡。野生型Hp-PRRSV在凋亡诱导的MARC-145细胞中的增殖研究表明，诱导凋亡进一步增加了病毒的产量，并在感染后的最初24 h内检测到病毒RNA的剧烈复制和病毒的快速释放。此外，当野毒株Hp-PRRSV在药物处理的Marc-145细胞中连续传代时，后代病毒在测试的世代中保持了稳定的病毒滴度和对其天然靶细胞的感染力。综上所述，这些发现表明，凋亡的MARC-145细胞对野生型Hp-PRRSV更敏感，病毒在这些细胞中的有效复制和细胞间传播促进了病毒的繁殖。(C)2015爱思唯尔B.V.保留所有权利。
Infection of porcine reproductive and respiratory syndrome virus (PRRSV) induces cell apoptosis both in vivo and in vitro. However, the correlation between host cell apoptosis and PRRSV replication is unclear. Here, the promotion of PRRSV propagation by cell apoptosis in MARC-145 cells was reported. The observation on propagation of field highly pathogenic PRRSV (HP-PRRSV) in MARC-145 cells showed that infection of overgrown MARC-145 cells obviously elevated virus production and cell apoptosis was triggered in these cells before virus inoculation. The investigation on propagation of field HP-PRRSV in apoptosis induced MARC-145 cells displayed that induction of apoptosis further increased the virus production and a vigorous viral RNA replication accompanied by fast virus release in these cells was detected in the initial 24 h post infection. In addition, when field HP-PRRSV was serially passed in drug-treated MARC-145 cells, the progeny viruses kept a stable viral titer and infectivity to its native target cells in the tested generations. In summary, these findings demonstrated that apoptotic MARC-145 cells were more susceptible to field HP-PRRSV and propagation of the virus was promoted by effective replication and cell-to-cell transmission of the virus in these cells. (C) 2015 Elsevier B.V. All rights reserved.