Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from bulip biomass.
Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from bulip biomass.
复制标题
从bulip生物质制备抗菌α-亚甲基-β-羟基-γ-丁内酯（tulipalin B）的环境友好方法。
DOI：
10.1080/09168451.2014.946395
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Y. Kato
中科院分区：
文献类型：
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作者：
T.Nomura;E. Hayashi;S. Kawakami;S. Ogita;and Y. Kato
郁金香红素B(α-亚甲基-β-羟基-γ-丁内酯，PAB)是一种存在于郁金香(Tulipa Gesneriana)中的抗菌天然产物。PAb是由前体6-郁金香苷B(PosB)在内源性转运酶(TCE)作用下直接合成的。尽管抗菌多克隆抗体在各种工业应用中具有潜在的应用价值，但缺乏简便的合成方案阻碍了其实际应用。在这里，我们描述了一种环境友好和简便的过程，使用郁金香生物质材料，基于TCE催化的一步酶反应，而不使用石油衍生的溶剂。通过对115个郁金香品种的筛选，我们发现了3个优良品种，它们几乎只在花组织中积累了PosB。花卉乙醇水提物用活性碳部分纯化后，与球茎粗提物制备的可重复使用的固定化三氯乙烷进行酶反应。反应在室温下几小时内完成，并用活性碳和乙醇分批提纯PAb。
Tulipalin B (α-methylene-β-hydroxy-γ-butyrolactone, PaB) is an antimicrobial natural product occurring in tulip (Tulipa gesneriana). PaB is directly formed from the precursor glucose ester 6-tuliposide B (PosB) by endogenous Pos-converting enzyme (TCE). Despite the potential usefulness of antibacterial PaB in various industrial applications, lack of facile synthetic schemes hampers its practical use. Herein, we describe an environmentally benign and facile process for the preparation of PaB using tulip biomass materials based on one-step enzyme reaction catalyzed by TCE without the use of petroleum-derived solvents. By screening 115 tulip cultivars, we found three elite cultivars, which accumulated PosB almost exclusively in flower tissues. The flower extracts with aqueous ethanol were partially purified with activated charcoal and subjected to the enzyme reaction with reusable immobilized TCE prepared from bulb crude extracts. The reaction was completed in a few hours at room temperature, and PaB was purified with activated charcoal and ethanol in a batch-wise manner.