ALTERATIONS IN THE ACCUMULATION OF ADENYLYLATED NUCLEOTIDES IN HEAVY-METAL-ION-STRESSED AND HEAT-STRESSED SYNECHOCOCCUS SP STRAIN PCC-6301, A CYANOBACTERIUM, IN LIGHT AND DARK
ALTERATIONS IN THE ACCUMULATION OF ADENYLYLATED NUCLEOTIDES IN HEAVY-METAL-ION-STRESSED AND HEAT-STRESSED SYNECHOCOCCUS SP STRAIN PCC-6301, A CYANOBACTERIUM, IN LIGHT AND DARK
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DOI：
10.1042/bj2760487
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发表时间：
1991-06-01
影响因子：
4.1
通讯作者：
BORBELY, G
中科院分区：
文献类型：
--
作者：
PALFI, Z;SURANYI, G;BORBELY, G
重金属离子-（Cd 2+、Cu 2+、Pb 2+、Hg 2+和Zn 2+）或热（50 ℃）-胁迫处理单细胞蓝细菌聚球藻，菌株PCC 6301在光照和黑暗条件下均导致双（5 '-核苷酰）寡磷酸的积累：Ap 4A、Ap 4G、Ap 3A、Ap 3G和Ap 3Gp 2。在光照条件下，Ap 4A和Ap 4G的积累是重金属离子应激细胞的更多特征，而Ap 3A、Ap 3G和Ap 3Gp 2的积累是能量剥夺期间（即在黑暗中）重金属离子或热休克处理的主要特征。这种双核苷寡磷酸的积累支持了这样一种模型，即腺苷酰化核苷酸是通过tRNA-氨酰合成酶的逆向反应合成的。这些核苷酸也可以在各种环境胁迫条件下转换或调节蓝藻的反应。
Heavy-metal-ion- (Cd2+, Cu2+, Pb2+, Hg2+ and Zn2+) or heat (50-degrees-C)-stress treatments of the unicellular cyanobacterium Synechococcus sp., strain PCC 6301, under both light and dark conditions led to the accumulation of bis(5'-nucleosidyl)oligophosphates: Ap4A, Ap4G, Ap3A, Ap3G and Ap3Gp2. Under light regimens, the accumulation of Ap4A and Ap4G is more characteristic of heavy-metal-ion-stressed cells, whereas the accumulation of Ap3A, Ap3G and Ap3Gp2 is the dominant feature of heavy-metal-ion or heat-shock treatment during energy deprivation (i.e. in the dark). This accumulation of bisnucleoside oligophosphates supports a model whereby the adenylylated nucleotides are synthesized by the backward reaction of tRNA-aminoacyl synthetases. These nucleotides may also act to switch or modulate cyanobacterial responses under various environmental stress conditions.