Ectopic bone formation during tissue-engineered cartilage repair using autologous chondrocytes and novel plasma-derived albumin scaffolds
Ectopic bone formation during tissue-engineered cartilage repair using autologous chondrocytes and novel plasma-derived albumin scaffolds
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DOI：
10.1016/j.jcms.2016.08.005
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发表时间：
2016-10-01
影响因子：
3.1
通讯作者：
Meana-Infiesta, Alvaro
中科院分区：
文献类型：
--
作者：
Costales, David Robla;Junquera, Luis;Meana-Infiesta, Alvaro
目的：本研究的目的有两个：首先，使用新型血浆源支架评估体外和体内软骨组织的产生，其次，测试使用这种方法对新西兰兔（NZr）耳部实验缺陷的修复。材料和方法：支架中接种软骨细胞，并在体外培养 3 个月，以检查体外软骨的产生。为了评估体内软骨的产生，将软骨细胞接种的支架皮下移植到裸鼠体内。为了检查体内修复情况，在五只新西兰兔子 (NZr) 的耳朵中制作的实验缺陷用软骨细胞接种的支架填充。结果：体外培养产生了不含细胞外基质 (ECM) 的成熟软骨细胞。体外再分化培养物的组织学检查显示分化的软骨细胞粘附在支架孔上。将这些构建体皮下移植到裸鼠中产生的软骨，经组织学研究证实。 5 NZr 的实验性软骨修复显示软骨组织修复了缺损，并与骨形成的钙化区域混合。结论：通过软骨细胞培养物和新型血浆衍生支架，可以在体内产生软骨组织并修复实验性耳廓缺损。需要进一步的研究来确定样本中骨形成的重要性。 (C) 2016 年欧洲颅颌面外科协会。由爱思唯尔有限公司出版。保留所有权利。
Purpose: The aims of this study were twofold: first, to evaluate the production of cartilaginous tissue in vitro and in vivo using a novel plasma-derived scaffold, and second, to test the repair of experimental defects made on ears of New Zealand rabbits (NZr) using this approach.Materials and methods: Scaffolds were seeded with chondrocytes and cultured in vitro for 3 months to check in vitro cartilage production. To evaluate in vivo cartilage production, a chondrocyte-seeded scaffold was transplanted subcutaneously to a nude mouse. To check in vivo repair, experimental defects made in the ears of five New Zealand rabbits (NZr) were filled with chondrocyte-seeded scaffolds.Results: In vitro culture produced mature chondrocytes with no extracellular matrix (ECM). Histological examination of redifferentiated in vitro cultures showed differentiated chondrocytes adhered to scaffold pores. Subcutaneous transplantation of these constructs to a nude mouse produced cartilage, confirmed by histological study. Experimental cartilage repair in five NZr showed cartilaginous tissue repairing the defects, mixed with calcified areas of bone formation.Conclusion: It is possible to produce cartilaginous tissue in vivo and to repair experimental auricular defects by means of chondrocyte cultures and the novel plasma-derived scaffold. Further studies are needed to determine the significance of bone formation in the samples. (C) 2016 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.