Multilayer Assembly of Tannic Acid and an Amphiphilic Copolymer Poloxamer 188 on Planar Substrates toward Multifunctional Surfaces with Discrete Microdome-Shaped Features
Multilayer Assembly of Tannic Acid and an Amphiphilic Copolymer Poloxamer 188 on Planar Substrates toward Multifunctional Surfaces with Discrete Microdome-Shaped Features
复制标题
单宁酸和两亲性共聚物泊洛沙姆 188 在平面基底上的多层组装，形成具有离散微圆顶形状特征的多功能表面
DOI：
10.1021/acs.langmuir.8b01982
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发表时间：
2018
期刊：
影响因子：
3.9
通讯作者：
Wei He
中科院分区：
文献类型：
--
作者：
Lianghong Peng;Fang Cheng;Yanan Zheng;Zengqian Shi;Wei He
单宁酸（TA）是一种天然多酚化合物，具有广泛的生物活性，其中最显着的是抗氧化活性。泊洛沙姆 188 (P188) 是一种聚环氧乙烷 - 聚环氧丙烷 - 聚环氧乙烷的合成三嵌段共聚物，本质上是两亲性的，以其密封结构受损的细胞膜的能力而闻名。将两种物质集成到平面基材上可以为多功能涂层带来新的选择，这对于可植入生物医学设备是有利的。在这里，我们证明了基于 TA 酚羟基和 P188 醚基之间的氢键，将 TA/P188 多层组装成这种涂层的可行性，以及它产生的独特表面特征。在溶液和基底支撑的逐层组装中研究了这两种化合物之间的相互作用。多层组装过程表现出指数增长模式，如紫外可见分光光度法和具有耗散的石英晶体微天平所表征。形态独特的微圆顶形表面特征随着组装层数的增加而出现和演变。这些特征为该涂层带来了储层功能，疏水性尼罗红染料的负载就证明了这一点。此外，通过硝酸银染色揭示了多层膜中TA的存在，并通过2,2-二苯基-1-三硝基苯肼自由基清除测定证明了其抗氧化活性。
Tannic acid (TA) is a natural polyphenol compound with a broad spectrum of biological activities, the most notable of which being antioxidation. Poloxamer 188 (P188), a synthetic triblock copolymer of poly(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide), is amphiphilic in nature and best known for its ability to seal structurally damaged cellular membranes. The integration of both substances onto planar substrates could bring a new option for multifunctional coatings that are advantageous for implantable biomedical devices. Here, we demonstrate the feasibility of multilayer assembly of TA/P188 toward such a coating based on hydrogen bonding between phenolic hydroxyls of TA and ether groups of P188, and the unique surface feature it generates. The interactions between these two compounds were studied both in solution and in substrate-supported layer-by-layer assembly. The multilayer assembly process exhibits an exponential growth pattern as characterized by UV–vis spectrophotometry and quartz crystal microbalance with dissipation. Morphologically unique, microdome-shaped surface features emerge and evolve with the number of layers assembled. Such features bring a reservoir function to this coating, as demonstrated by the loading of hydrophobic nile red dye. Furthermore, the presence of TA in the multilayers was revealed by silver nitrate staining, and its antioxidation activity was demonstrated through a 2,2-diphenyl-1-picryl-hydrazyl free-radical scavenging assay.