Serum metabonomics of NAFLD plus T2DM based on liquid chromatography-mass spectrometry
Serum metabonomics of NAFLD plus T2DM based on liquid chromatography-mass spectrometry
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基于液相色谱-质谱法的 NAFLD 合并 T2DM 血清代谢组学
DOI：
10.1016/j.clinbiochem.2016.05.016
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发表时间：
2016-09-01
影响因子：
2.8
通讯作者：
Feng, Rennan
中科院分区：
文献类型：
--
作者：
Chen, Yang;Li, Chunlong;Feng, Rennan
目的：非酒精性脂肪性肝病(NAFLD)是世界范围内的主要肝病，与胰岛素抵抗、2型糖尿病(T2 DM)等代谢性疾病密切相关。本研究的目的是确定有或没有T2 DM的NAFLD患者的不同代谢物。设计和方法：我们使用生物标记物发现群体来寻找有或没有T2 DM的NAFLD患者的不同代谢物。然后，应用验证总体来检验生物标记物发现总体的模型。所有受试者都接受了人体测量和普通生化测量。结果：在电喷雾电离(ESI)模式下，有7，7，2种代谢物在生物标志物发现群体和验证群体之间被鉴定。非酒精性脂肪肝患者血清油酰胺、L-苯丙氨酸、L-脯氨酸、胆红素、L-棕榈酰肉碱和PC(20：5)浓度高于对照组，而Lyso-PAF-C-18浓度低于对照组。与对照组比较，非酒精性脂肪肝+2型糖尿病组血清油酰胺、L亮氨酸、溶血磷胆碱(14：0)、胆红素、十四烯酰肉碱、亚油酰肉碱、十四碳二烯卡尼汀均显著升高。结论：血清代谢组学分析显示NAFLD及NAFLD合并T2 DM患者存在明显的代谢改变。本研究有助于进一步探讨NAFLD及NAFLD合并T2 DM的发病机制及病理生理机制，为NAFLD及NAFLD合并T2 DM的防治提供参考。(C)2016年加拿大临床化学家协会。爱思唯尔公司出版，版权所有。
Objectives: Nonalcoholic fatty liver disease (NAFLD), a main liver disease around the world, is closely associated with insulin resistance, type 2 diabetes mellitus (T2DM) and other metabolic diseases. The objective of this study is to identify distinct metabolites of NAFLD patients with or without T2DM.Design and methods: We used a biomarker-discovery population to find distinct metabolites of NAFLD patients with or without T2DM. Then, a validation population was applied to test the model of the biomarker-discovery population. All the individuals received anthropometric and common biochemical measurements. The metabolic data were analyzed by multivariable statistical analyses using ultra-high-performance liquid chromatography/quadrupole time-of-flight-tandem mass spectrometry.Results: There were 7,7,2 metabolites in the positive electrospray ionization (ESI) mode, which were identified between groups from both the biomarker-discovery and validation population. The NAFLD group showed higher concentrations of oleamide, L-phenylalanine, L-proline, bilirubin, L-palmitoylcarnitine, and PC (20:5) and a lower concentration of Lyso-PAF C-18 than those of control. Compared with the control group, the NAFLD + T2DM group displayed higher oleamide, L-leucine, LysoPC (14:0), bilirubin, tetradecenoylcarnitine, linoleyl carnitine, and tetradecadiencarnitine in serum. Tetradecenoylcarnitine and tetradecadiencarnitine were more elevated in patients with NAFLD + T2DM than in the NAFLD group.Conclusions: Serum metabonomic analyses displayed great metabolic changes in patients with NAFLD and NAFLD plus T2DM. Our study is beneficial in providing a further view into the pathogenesis and pathophysiology of NAFLD and NAFLD plus T2DM, which might be useful for the prevention and therapy of NAFLD and NAFLD plus T2DM. (C) 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.