INFLUENCE OF SURFACE TEXTURE OF POLYMERIC SHEETS ON PERIPHERAL-NERVE REGENERATION IN A 2-COMPARTMENT GUIDANCE-SYSTEM
INFLUENCE OF SURFACE TEXTURE OF POLYMERIC SHEETS ON PERIPHERAL-NERVE REGENERATION IN A 2-COMPARTMENT GUIDANCE-SYSTEM
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DOI：
10.1016/0142-9612(91)90210-2
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发表时间：
1991-03-01
期刊：
影响因子：
14
通讯作者：
AEBISCHER, P
中科院分区：
文献类型：
--
作者：
GUENARD, V;VALENTINI, RF;AEBISCHER, P
合成引导通道是研究周围神经再生机制的有用工具。 在本研究中，硅胶弹性体管的管腔被沿着管长度放置的10 mm长的聚合物条带分为两个隔室。 分析了改变亲水性和疏水性聚合物条带的表面纹理对再生神经组织形态的影响。 使用具有光滑（S-NC和S-PVDF）或粗糙（R-NC、R-PVDF）表面纹理的亲水性硝化纤维素（NC）和疏水性聚偏氟乙烯（PVDF）膜。 每种类型的5个通道用于修复大鼠坐骨神经横断，并在4周后进行分析。 组织条桥接所有R-NC和R-PVDF管中的神经残端，5个S-NC管和3个S-PVDF管中的神经残端。 在R-NC和R-PVDF管中，观察到钟形组织粘附到聚合物条，而在S-NC和S-PVDF管中，观察到圆形的自由漂浮的神经电缆。 所有电缆都含有有髓和无髓轴突以及聚集在微束中并被神经外膜层包围的许旺细胞。 对于两种粗糙条，初始细胞层由粘附在聚合物表面的巨噬细胞组成。 与NC条相比，PVDF的接触粗糙表面的神经外膜神经组织明显更薄。 对于面向硅胶管或光滑NC和PVDF条的神经，未观察到神经外膜厚度差异。 S-PVDF管比S-NC管含有显著更多的有髓鞘轴突。 这项研究表明，再生的周围神经的形态显着影响的引导通道内放置的带的表面纹理。 再生神经组织和用作神经引导通道的聚合物材料之间的相互作用可用于控制再生神经的模式和形态。
Synthetic guidance channels are useful tools to study the mechanisms underlying peripheral nerve regeneration. In the present study, the lumen of silicone elastomer tubes was divided into two compartments by a polymer strip 10 mm long placed along the tube length. The influence of varying the surface texture of hydrophilic and hydrophobic polymer strips on the morphology of the regenerated neural tissue was analysed. Hydrophilic nitrocellulose (NC) and hydrophobic polyvinylidene fluoride (PVDF) films with smooth (S-NC and S-PVDF) or rough (R-NC, R-PVDF) surface texture were used. Five channels of each type were used to repair transected rat sciatic nerves and analysed after 4 wk. Tissue strips bridged the nerve stumps in all R-NC and R-PVDF tubes, in five of the S-NC and three of the S-PVDF tubes. In R-NC and R-PVDF tubes, bell-shaped tissue adhering to the polymer strip was observed, whereas in S-NC and S-PVDF tubes round, free-floating nerve cables were seen. All the cables contained myelinated and unmyelinated axons and Schwann cells grouped in microfascicles and surrounded by an epineurial layer. For both rough strips, the initial cell layer consisted of macrophages adhering to the polymer surface. The epineurial nerve tissue contacting the rough surface was significantly thinner for PVDF compared with NC strips. No difference in epineurial thickness was observed for nerves facing the silicone tube or for smooth NC and PVDF strips. S-PVDF tubes contained significantly more myelinated axons than S-NC tubes. This study shows that the morphology of regenerated peripheral nerves is significantly influenced by the surface texture of strips placed within guidance channels. Interactions between regenerating neural tissue and polymeric materials used as nerve guidance channels may be useful in controlling patterns and morphologies of regenerating nerves.