Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana.
Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana.
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DOI：
10.1007/978-1-4939-9635-3_3
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发表时间：
2019
影响因子：
--
通讯作者：
Y. Sato;Hideki Takahashi
中科院分区：
文献类型：
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作者：
Y. Sato;Hideki Takahashi
抗病毒RNA沉默和抗病基因诱导的防御反应是植物主要的抗病毒免疫系统。几个相关的成分已经在拟南芥中被遗传或生化鉴定。剖析抗病毒免疫系统的一个强有力的工具是一种反向遗传学方法，它可以分析抗病毒防御反应受损的突变株系。特别地，为了更好地理解参与宿主植物中抗性基因赋予的抗病毒应答的信号传导网络，需要建立携带纯合突变等位基因和抗病毒抗性基因的突变系。本章提供的关于防御相关信号突变等位基因的信息和基于PCR的基因分型方法可以有效地选择可用于研究抗病毒抗性信号网络和抗性机制的突变株系。
Antiviral RNA silencing and the resistance gene-conferred defense response are major antiviral immune systems in plants. Several of the components involved have been genetically or biochemically identified inArabidopsis thaliana. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzesArabidopsismutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the resistance gene-conferred antiviral response in host plants, establishment of mutant lines carrying the homozygous mutant allele and antiviral resistance gene is required. The information on well-characterized defense-related signaling mutant alleles and the PCR-based genotyping method provided in this chapter allows the efficient selection ofArabidopsismutant lines that can be used to study antiviral resistance signaling networks and resistance mechanisms.