Analysis of the effects of an orifice plate on the membrane potential in electroporation and electrofusion of cells
Analysis of the effects of an orifice plate on the membrane potential in electroporation and electrofusion of cells
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DOI：
10.1088/0022-3727/40/6/036
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发表时间：
2007-03-21
影响因子：
3.4
通讯作者：
Washizu, Masao
中科院分区：
文献类型：
--
作者：
Techaumnat, Boonchai;Washizu, Masao
本文提出了一种数值分析的膜电压感应生物细胞，在外部施加的电场的影响下，如电穿孔或电融合的过程。我们专注于配置，其中使用的绝缘板与孔和研究的情况下（a）一个细胞放置在孔或（B）两个细胞在孔接触，当一个步进电压施加在整个板。基于边界元法的配方是假设生物膜是一个无限薄的绝缘体。计算结果表明，由于孔板产生的场收缩，几乎所有的外加电压都施加在孔处的膜上，孔外的膜电压几乎为零;使用孔板进行场剪裁可以控制膜电压的大小和位置。它还表明，我们可以诱导击穿专门围绕一对细胞的接触点，高产电融合与这样的几何形状。
This paper presents a numerical analysis of the membrane voltage induced on biological cells, under the influence of an externally applied field in such processes as electroporation or electrofusion. We focus on the configurations in which an insulator plate with an orifice is used and study the cases of (a) a cell placed on the orifice or (b) two cells in contact at the orifice, when a stepwise voltage is applied across the plate. Formulation based on the boundary element method is made assuming that a biological membrane is an infinitesimally thin insulator. Results of the calculation show that, due to the field constriction created by the orifice plate, almost all the externally applied voltage is imposed on the membrane at the orifice, and the membrane voltage outside the orifice is virtually zero; field tailoring with the use of the orifice plate enables control over the magnitude and localization of the membrane voltage. It is also shown that we can induce breakdown exclusively around the contact point of a pair of cells for high-yield electrofusion with such a geometry.