CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage
CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage
复制标题
细胞周期开关 52 通过与不结球白菜侧向抑制子相互作用来调节分蘖
DOI：
10.1016/j.plantsci.2021.110934
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发表时间：
2021-05-15
期刊：
影响因子：
5.2
通讯作者：
Li, Ying
中科院分区：
文献类型：
--
作者：
Guo, Mingliang;Long, Yan;Li, Ying
随着水稻MONOCULM 1（MOC1）和拟南芥LAS（LATERAL SUPPLYOR）、番茄LS（LATERAL SUPPLYOR）等调控分蘖的重要基因的发现，对分蘖机制的研究取得了很大进展，但对不结球白菜（NHCC）分蘖的研究还很少。在这里，我们报告BcLAS，作为GRAS家族的成员，在NHCC营养生长期间的分蘖中起着重要作用。BcLAS在除叶腋外的其它部位几乎不表达。利用病毒诱导的基因沉默（VIGS）技术沉默BcLAS基因的表达后，我们发现'玛尔头'的分蘖数急剧下降。在‘苏州青’中，BcLAS的过量表达显著促进了分蘖。BcCCS 52是细胞周期启动子52（CCS 52）的直向同源物，与BcLAS相互作用。下调BcCCS 52的表达促进了“苏州青”的分蘖，因此，BcCCS 52在分蘖调控中起负作用。我们的研究结果揭示了营养生长期NHCCs的分蘖调控机制，并报道了NHCCs中调控分蘖的CCS52的直向同源物。
With the discovery of essential genes regulating tillering, such as MONOCULM 1 (MOC1) in rice and LATERAL SUPPRESSOR (LAS in Arabidopsis, LS in tomato), research on tillering mechanisms has made great progress; however, the study of tillering in non-heading Chinese cabbage (NHCC) is rare. Here, we report that BcLAS, as a member of the GRAS family, plays an important role in the tillering of NHCC during its vegetative growth. BcLAS was almost not expressed in other examed parts except leaf axils throughout life. When the expression of BcLAS was silenced utilizing virus-induced gene silencing (VIGS) technology, we found that the tiller number of 'Maertou' decreased sharply. In 'Suzhouqing', overexpression of BcLAS significantly promoted tillering. BcCCS52, the orthologue to CELL CYCLE SEITCH 52 (CCS52), interacts with BcLAS. Downregulation of the expression of BcCCS52 promoted tillering of 'Suzhouqing'; therefore, we conclude that BcCCS52 plays a negative role in tillering regulation. Our findings reveal the tillering regulation mechanism of NHCCs at the vegetative stage and report an orthologue of CCS52 regulating tillering in NHCC.