Immunisation with phage displaying peptides representing single epitopes of the glycoprotein G can give rise to partial protective immunity to HSV-2
Immunisation with phage displaying peptides representing single epitopes of the glycoprotein G can give rise to partial protective immunity to HSV-2
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DOI：
10.1006/viro.2000.0185
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发表时间：
2000-03-30
期刊：
影响因子：
3.7
通讯作者：
Irving, WL
中科院分区：
文献类型：
--
作者：
Grabowska, AM;Jennings, R;Irving, WL
将代表HSV-2（gG 2）的糖蛋白G的单个表位的丝状噬菌体展示肽作为免疫原，通过皮下途径在Balb/c小鼠中使用，而不使用额外的佐剂。从随机噬菌体肽展示文库中分离噬菌体，并含有模拟gG 2表位的15-mer肽插入物。在每种情况下，产生对gG 2的抗体应答，其取决于施用的噬菌体的剂量和肽插入物的存在。噬菌体展示gG 2的表位，其定位于氨基酸551-570，是最具免疫原性的;有趣的是，gG 2的该区域经常被感染HSV-2的患者识别。这些数据还提供了关于选择肽模拟物用作免疫原的有趣信息，因为。令人惊讶。最具抗原性的单个克隆是免疫原性最低的。在两个实验中，用展示gG 2的单个表位的噬菌体免疫的小鼠被保护免于用致死剂量的完整HSV-2攻击。这表明噬菌体展示肽在诱导针对病原体的保护性免疫中的可能作用，并为研究潜在机制提供了模型系统。(C)北京大学出版社.
Filamentous phage displaying peptides representing single epitopes of the glycoprotein G of HSV-2 (gG2) were used as immunogens via the subcutaneous route in Balb/c mice without additional adjuvant. The phage were isolated from a random phage peptide display library and contain 15-mer peptide inserts that mimic epitopes of gG2. In each case, an antibody response to gG2 was generated that was dependent on the dose of phage administered and on the presence of the peptide insert. Phage displaying epitopes of gG2, which map to amino acids 551-570, were the most immunogenic; interestingly, this region of gG2 is frequently recognised by patients infected with HSV-2. The data also provide interesting information as regards choice of peptide mimics for use as immunogens because. surprisingly. the most antigenic of the individual clones was the least immunogenic. In two of the experiments, mice immunised with phage displaying a single epitope of gG2 were protected against challenge with a lethal dose of whole HSV-2. This suggests a possible role for phage-displayed peptides in inducing protective immunity against pathogens and provides a model system for investigating the underlying mechanisms. (C) 2000 Academic Press.