Nanoparticles with Aggregation-induced Emission for Monitoring Long Time Cell Membrane Interactions
Nanoparticles with Aggregation-induced Emission for Monitoring Long Time Cell Membrane Interactions
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DOI：
10.2528/pier13040212
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发表时间：
2013
影响因子：
6.7
通讯作者：
Hao Cheng;Wei Qin;Zhenguo Zhu;J. Qian;A. Qin;B. Tang;Sailing He
中科院分区：
文献类型：
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作者：
Hao Cheng;Wei Qin;Zhenguo Zhu;J. Qian;A. Qin;B. Tang;Sailing He
我们以高空间和时间分辨率对纳米颗粒-细胞膜相互作用进行长时间监测。将2, 3-双(4-(苯基1(4-(1, 2, 2-三苯基乙烯基)苯基)氨基)苯基)富马腈(TPE-TPA-FN)掺杂在有机改性二氧化硅(ORMOSIL)中形成生物相容性纳米探针，表现出聚集诱导发射(ATE)效应。测试了这种合成纳米颗粒的光漂白抗性，并与同类纳米颗粒进行了比较，证明了其优越性和长期荧光发射的能力。我们利用基于物镜的全内反射显微镜结合活细胞孵化平台来实时研究这种纳米粒子的细胞摄取过程。
We perform the long time monitoring of nanoparticle-cell membrane interaction with high spatial and temporal resolution. The 2, 3-bis(4-(pheny1(4-(1, 2, 2-triphenylvinyl) phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN) is doped in organically modified silica (ORMOSIL) to be a biocompatible nanoprobe, which displays an aggregation-induced emission (ATE) effect. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of long term fluorescence emission. We utilize the objective-based total internal reflection microscopy combined with the living cell incubation platform to investigate the cell uptake process of this nanoparticle in real time.