A growing microcolony can survive and support persistent propagation of virulent phages
A growing microcolony can survive and support persistent propagation of virulent phages
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DOI：
10.1073/pnas.1708954115
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发表时间：
2018-01-09
影响因子：
11.1
通讯作者：
Mitarai, Namiko
中科院分区：
文献类型：
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作者：
Eriksen, Rasmus Skytte;Svenningsen, Sine L.;Mitarai, Namiko
细菌形成菌落并分泌细胞外聚合物包围单个细胞。这些空间结构通常与细菌之间的协作和群体感应有关。在这里，我们调查的相互保护提供的球形生长的单克隆菌落暴露在其表面上增殖的细菌。作为概念的证明，我们将大肠杆菌的生长菌落暴露于噬菌体P1的毒性突变体。当殖民地由少于50，000个相似的成员组成时，其被消除，而较大的初始菌落允许噬菌体抗性突变体以及重要的是，大多数噬菌体敏感成员的菌落长期存活。一个数学模型预测，仅由噬菌体敏感的细菌形成的菌落可以存活，因为细菌在整个殖民地的生长超过了表面细菌的杀灭，并精确地指出了临界菌落大小如何取决于噬菌体感染周期中的关键参数。
Bacteria form colonies and secrete extracellular polymeric substances that surround the individual cells. These spatial structures are often associated with collaboration and quorum sensing between the bacteria. Here we investigate the mutual protection provided by spherical growth of a monoclonal colony during exposure to phages that proliferate on its surface. As a proof of concept we exposed growing colonies of Escherichia coli to a virulent mutant of phage P1. When the colony consists of less than similar to 50,000 members it is eliminated, while larger initial colonies allow long-term survival of both phage-resistant mutants and, importantly, colonies of mostly phage-sensitive members. A mathematical model predicts that colonies formed solely by phage-sensitive bacteria can survive because the growth of bacteria throughout the colony exceeds the killing of bacteria on the surface and pinpoints how the critical colony size depends on key parameters in the phage infection cycle.