Translation of globin messenger RNA in a heterologous cell-free system.
Translation of globin messenger RNA in a heterologous cell-free system.
复制标题
球蛋白信使 RNA 在异源无细胞系统中的翻译。
作者：
M. Mathews;M. Osborn;J. Lingrel
信使特异性启动因子能够区分病毒RNA中信使RNA的类别(MRNAs)或不同的顺反子，参与了细菌1-5中蛋白质合成的调节。在真核系统6-10中也进行了类似但不太详细的观察。例如，从哺乳动物病毒(脑心肌炎病毒，EMC)中提取的RNA不能在无网织红细胞系统中翻译，除非反应细胞的提取物加强该系统-在这种情况下，Krebs II腹水细胞6。这些结果暗示了组织特异性因子的存在，并引发了这样的疑问：这种不相容是否是由于Krebs II腹水细胞系统无法对珠蛋白的mRNA做出反应而导致的。
MESSENGER-SPECIFIC initiation factors, capable of discriminating between classes of messenger RNAs (mRNAs) or different cistrons in viral RNA, have been implicated in the regulation of protein synthesis in bacteria1–5. Comparable but less detailed observations have also been made in eukaryotic systems6–10. For example, RNA extracted from a mammalian virus (encephalomyocarditis virus, EMC) cannot be translated in a reticulocyte cell-free system unless the system is fortified with an extract from responsive cells—in this case, Krebs II ascites cells6. Such results imply the existence of tissue-specific factors and lead to questions whether this incompatibility is reciprocated by an inability of the Krebs II ascites cell system to respond to the mRNA for globin.