5-Hydroxytryptophan: an absorption and fluorescence probe which is a conservative replacement for [A14 tyrosine] in insulin.
5-Hydroxytryptophan: an absorption and fluorescence probe which is a conservative replacement for [A14 tyrosine] in insulin.
复制标题
5-羟基色氨酸：一种吸收和荧光探针，是胰岛素中 [A14 酪氨酸] 的保守替代品。
DOI：
10.1007/bf01886763
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ross,JB
中科院分区：
文献类型：
--
作者：
Laws,WR;Schwartz,GP;Rusinova,E;Burke,GT;Chu,YC;Katsoyannis,PG;Ross,JB
利用胰岛素固有的酪氨酸吸收和荧光来监测其与胰岛素受体的相互作用是有限的，因为受体色氨酸残基的光谱特性掩盖了激素酪氨酸残基的光谱特性。我们描述了一种胰岛素类似物的合成，其中A14酪氨酸被色氨酸类似物5-羟色氨酸取代。这种胰岛素是光谱增强的，因为5-羟色氨酸有一个300 nm以上的吸收带，其能量低于色氨酸的吸收。稳态和时间分辨荧光参数表明，5-羟色氨酸报告的关于A14侧链环境的信息与相应的含色氨酸的胰岛素相同。与猪胰岛素相比，合成激素是一种完全的激动剂，但比活性略有降低。因此，这种光谱增强的胰岛素类似物将对激素-受体相互作用的研究有用，因为即使在含有色氨酸的受体存在的情况下，它也可以通过吸收和荧光观察到。
Use of insulin's intrinsic tyrosine absorption and fluorescence to monitor its interaction with the insulin receptor is limited because the spectral properties of the receptor tryptophan residues mask the spectral properties of the hormone tyrosine residues. We describe the synthesis of an insulin analog where A14 tyrosine is replaced by a tryptophan analog, 5-hydroxytryptophan. This insulin is spectrally enhanced since 5-hydroxytryptophan has an absorption band above 300 nm which is at lower energies than the absorption of tryptophan. Steady-state and time-resolved fluorescence parameters indicate that 5-hydroxytryptophan reports the same information about the environment of the A14 side chain as does the corresponding tryptophan-containing insulin. The synthetic hormone is a full agonist compared to porcine insulin, but has slightly reduced specific activity. Consequently, this spectrally enhanced insulin analog will be useful for hormone-receptor interaction studies since it can be observed by both absorption and fluorescence even in the presence of the tryptophan-containing receptor.